Capillary Electrophoresis Analysis and Japanese Population Data on CSF1PO Locus
Kentaro Kasai, Koji
Fujii, Kanako Yoshida, Kazumasa Sekiguchi, Hiroaki Senju and Hajime Sato
National Research Institute of Police Science, Tokyo, Japan
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PCR based STR typings are widely investigated for the forensic DNA typing from degraded samples such as aged bloodstains, decomposed tissues and so on. We reported on the last symposium that the CSF1PO types were detected using new detection method from bloodstain samples left over 16 years whose types were not able to be detected by the conventional system. Newly designed primers for this method reduced the amplified sizes 145 bp shorter than conventional ones do.
In this report, capillary electrophoresis using ABI PRISMÔ 310 Genetic Analyzer and polymer (POP4) was verified to detect CSF1PO locus with high sensitivity and reproducibility. We demonstrated in a recent meeting of this symposium that internal allelic ladder was needed to distinguish one base difference such as allele 10 and allele 10-1 (9.3) of TH01 locus when gel fragment analyzer system (ABI 373A) was used. However, standard deviations of base length data of each allele on CSF1PO locus collected using the ABI 310 capillary system were ranged from 0.0619 bp to 0.0911 bp. These values mean that internal allelic ladder was no longer needed to analyze STR and high reproducibility was achieved using this system.
Allele frequency data on CSF1PO locus were also collected from 307 Japanese. Allele 12 has the highest frequency of 0.397. Expected Heterozygosity and Power of Discrimination were 0.730 and 0.873, respectively.
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