Detection of STR, D1S80 and Gender Alleles from Body Fluid Collected on IsoCode Foam Collectors and Paper-based Devices
Reena Roy, Ph.D.,1,3
Robert Burghoff, Ph.D.,2 and David L. Steffens, Ph.D.3
1Nebraska State Patrol Criminalistics Laboratory, Lincoln, NE 68504
2Schleicher & Schuell, Inc., Keene, NH 03431
3LI-COR, Inc., Biotechnology Division, Lincoln, NE 68504
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The analysis of short tandem repeat (STR) and variable number of tandem repeats (VNTR) polymorphism is used extensively in forensic analysis, paternity testing and gene mapping. In the current research, DNA stored on IsoCodeÔ Stix and Prototype 2b paper-based devices obtained from Schleicher and Schuell, Inc. (Keene, NH) was subsequently amplified and analyzed by a "paper-in" amplification procedure. Saliva was collected on foam devices from the same manufacturer and transferred to the paper devices. After drying, Stix triangle or the Prototype 2b paper was rinsed three times with sterile distilled water. Rinsed pieces were placed directly into the amplification reaction and amplified using polymerase chain reaction (PCR) technology.
STR, D1S80 and gender alleles were detected using either an infrared (IR) fluorescent DNA sequencer or silver stain technology. Preparation of template DNA for amplification took only approximately five minutes. Amplification and detection of the STR, D1S80 and gender alleles using the infrared automated DNA sequencer was accomplished within one day.
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