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DNA was extracted from blood samples of 316 unrelated individuals living in Western Austria using the phenol-chloroform method. Small amounts of template DNA (1-3ng) were amplified for the short tandem repeat locus CD4 and separated on a 373 Stretch Sequenzer (ABD). 8 alleles were identified within the sample population, the allele frequencies showing similar results to other reported white Caucasian data. Exact test and Chi-square test showed no deviation from Hardy-Weinberg expectations. The discrimination power was 0,699. Dilution series down to 50 pg of DNA were amplified giving the same results as the undiluted sample. No allelic drop-out occurred. The data demonstrate that CD4 is a highly sensitive marker and a useful tool for forensic identity testing.