Time-of-Flight Mass Spectrometry: An Effective Tool for High-Throughput DNA Analysis
John M. Butler, Thomas Shaler,
Yuping Tan, Jia Li, Hua Lin, Joanna Hunter, Dan Pollart, Gordon Haupt, Joseph Monforte,
and Christopher Becker
GeneTrace Systems, Inc., 333 Ravenswood Avenue, Menlo Park, CA 94025
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Rapid analysis of DNA fragments may now be conducted using time-of-flight mass spectrometry, which offers at least two orders of magnitude faster separation times than any electrophoretic method. With parallel preparation of samples on a robotic workstation, thousands of samples may be processed daily. Reliable analysis of multiplexed short tandem repeats (STRs), multiplexed single nucleotide polymorphisms (SNPs), and DNA sequencing reactions will be shown. The possibilities of sequencing mitochondrial DNA or detecting point mutations in polymorphic regions of the genome, including clear separation of heterozygotes, will be discussed.
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