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A Computerized Method for the Collection of Quality Control Data in DNA Analysis in the RCMP

 

Sylvain A. Lalonde
Biology Section, Forensic Laboratory Edmonton, Royal Canadian Mounted Police

 

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The Royal Canadian Mounted Police Forensic Laboratory Edmonton began forensic RFLP DNA analysis in January, 1993. Each casework gel contains a male cell line, female cell line and Blood Internal Standard (BIS) for QC purposes. The data was originally collected in log books but was soon converted to a dBase IV® collection system. In early 1994, a Windows 3.X® program (DNA Stats©) was written and used to collect the data in a dBase IV® compatible file. As well as collecting the data, DNA Stats© also produces a report on the Measurement Imprecision of the samples being entered. A series of dBase IV® programs were written to tabulate the mean, median, standard deviation and relative deviation, largest and smallest allele measured for each allele using D1S7, D2S44, D5S110, D10S28, D16S85 and D17S79 plus the same for the monomorphic loci DYZ1 and D7Z2 for each sample. These results have been tabulated starting in January, 1993 and a history of results from the Forensic Laboratory Edmonton is presented. The collection of data from the Edmonton laboratory along with those in the other five (5) RCMP forensic laboratories has been used to establish the expected values for each allele. An examination of the medians and the relative deviations show the results have been extremely consistent over time, confirming the consistency and robustness of the RCMP RFLP protocol.


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