Melanie Richard, Pamela Newall, and Wayne Murray
Centre of Forensic Sciences, Toronto, Ontario, Canada

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Since 1994, we have successfully processed and reported more than 500 cases using the Forensic Science Service 7Quadruplex (vWA, TH01, F13A01, FES/FPS) in an automated fluorescence detection format. Multiplex STR analysis of mixed and degraded samples has yielded interpretable and significant results. Integral to the successful interpretation of mixtures is the potential for quantitative assessment of the components. However, the frequency of common alleles at STR loci may preclude the unequivocal allocation of genotypes usually possible in RFLP interpretation of multi-source mixtures and thus necessitate STR analysis at additional loci. Successful amplification and analysis of 1 nanogram or less of target DNA with the 10 locus multiplex, AmpFISTR ProfilerÔ from Applied Biosystems Division not only conserves case material but also substantially reduces the time required to generate extremely informative results. Results from several cases, including severely challenged samples such as spermatozoa from a sink trap and commonly encountered epithelial mixtures such as fingernail scrapings, amplified with a multiplex of Amelogenin, D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317, D7S820 (AmpFISTR ProfilerÔ) will be presented and interpreted.