Effect of Time and Environmental Factors on Postmortem Stability of DNA in Egypt
Mona M. Hassan1, Ragaa T.
Darwish1, and Dalal M. El-Kaffash2
1Forensic Medicine and Toxicology Dept,
2Clinical Pathology Dept., Alexandria University; Faculty of Medicine, Egypt
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The applicability of DNA polymorphism to the individualization of human blood and tissues have been demonstrated. DNA may degrade rapidly in cadaver’s blood and tissues even in the early post mortem period. This might be a consequence of rapid bacterial growth in decomposing bodies. High humidity conditions and temperatures are factors that affect the degradation rate of DNA. Our study aimed at evaluating the DNA integrity of muscle tissue in different environmental conditions in Egypt within different time intervals. DNA extraction was done using Wizard® Genomic DNA purification system. Restriction enzyme digestion of DNA were EcoRI, SmaI, Hind III (Boehringer Mannheim).
DNA gel electrophoresis with ethidium bromide was used to visualize DNA pattern followed by Southern Blotting application and hybridization. Our results and photos will be presented and discussed in full detail.
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