Sex Typing of Different Human Samples Using X and Y Specific Probes by in

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Sex Typing of Different Human Samples Using X and Y Specific Probes by in-situ Hybridization

Dalal M. El-Kaffash¹, Ragaa T. Darwish², and Mona M. Hassan²
¹Clinical Pathology Dept
²Forensic Medicine and Toxicology Dept, Alexandria University, Faculty of Medicine, Egypt

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Diagnosing the sex of biological evidence can provide important information in a forensic investigation. Many methods for determining the human male have been developed basically with the use of chromosome Y-specific sequences. In this study we used in situ hybridization technique for sexing human cells from tissues and biological fluids randomly collected and blindly tested. The hybridization signals were compared by testing the probes on fresh blood smears and male metaphase spread. Our results will be discussed in detail.

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