Ugo Ricci, S. Felici
Criminal Laboratory Department, Florence, Italy

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In a forensic laboratory, the treatment of pieces of crime evidence frequently involves using different techniques for the samples, to put the different elements in evidence. When we examine envelopes, stamps, pieces of plastic, or cigarette butts, it is possible to notice in a first phase, the presence of a latent fingerprint using the ninhydrin method. Afterwards, we apply the polymerase chain reaction (PCR) to investigate the HLA DQ alpha Gene, or other systems of dot-blot such as LDLR, GYPA, D7S8, or GC ED HBGG, which are present in a Polymarker^® commercial kit. We are now studying the effects of ninhydrin, DFO, DFO-ninhydrin, Cyanoacrilate and laser methods on evidence samples to value the efficiency of the GenePrint^™ STR Systems after these specific latent fingerprint applications.

In this communication, we report the preliminary results of the investigation of some samples of envelopes with bloodstains of donors for 7 STR (FESFPS, vWA, F13B, CSF1PO, F13A01, TH01 and TPOX). The envelopes were first processed with ninhydrin in 1,1,2-triclotrifluoroethan, with heat and steam. Subsequently, the bloodstains were removed and the DNA extracted with a non-organic procedure and amplified by PCR with monoplex system listed up. It seems that the amplification and typing of the samples show a good efficiency for the employed systems.