Steven D. Creacy, Charles M. Kelly and Robert A. Bever
Genetic Design, Inc., Greensboro, NC

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Eight STR loci (CSF1PO, TPOX, TH0I, VWA, F13A, F13B, LIPOL, FESFPS), which are commercially available, have an average combined power of exclusion of greater than 99.5% and an average Combined Paternity Index of greater than 100:1. However, more loci are needed to resolve those cases which do not meet the requisite paternity index or number of exclusions. The STR locus D19S253 has been developed to supplement the eight commercially available STR loci. Sequence analysis of D19S253 showed that it is a single tetranucleotide tandem repeat. One hundred Black, Caucasian and Hispanic exclusionary paternity cases were analyzed to determine the power of exclusion (approximately 65%), heterozygosity values (approximately 78%), and allele frequencies for this locus. Database analysis revealed that the locus consists of at least eleven alleles of five to fifteen repeats. Statistical analysis of the population databases demonstrates that D19S253 fits Hardy-Weinberg expectations and is in linkage equilibrium with the other eight STR loci.