Jaroslaw A. Berent, Marcin Wozniak and Danuta Miscicka-Sliwka
Forensic Medicine Institute, The Ludwik Rydygier University School of Medical Sciences, Bydgoszcz, Poland

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The method of calculation of the paternity probability for a hexaplex PCR reaction is presented is this paper.

Results of routine serological tests performed in our Institute for 300 unrelated persons were investigated. PCR reactions consisted of the following microsatellite loci: D1S103, TH01, D21S11, D18S51, FGA and amelogenin for sex determination. Primers were fluorescein labeled in 5' position and the amplification products were separated by electrophoresis on an A.L.F. Sequencer (Pharmacia). Fragment Manager computer software was used to determine the fragment length on the base of the external and internal markers. Because of the lack of the appropriate allelic ladders, every DNA band was allocated to arbitrarily defined intervals and then their frequencies were calculated.

The population frequencies provided the data for the following calculation of the paternity probability. The well-known method by Essen-Möller was adopted using our own algorithm which allows its application to any discrete serological system (systems with a defined number of codominant alleles). The computer program compiled in Turbo Pascal 7.0 (Borland) was developed to perform all calculations. This program can be modified easily for any other multiplex system.

The results of calculation of the paternity probability based on the investigated hexaplex system for the example data were confirmed to values found for classical serological systems as well as for loci D1S80, DQA1, PolyMarker and multilocus systems.