Jeanne M. Willard, MFS, Demris A. Lee, MSFS, Mitchell M. Holland, PhD, LTC Victor W.
Weedn, MC, USA
The Armed Forces DNA Identification Laboratory, Office of the Armed Forces Medical
Examiner, Armed Forces Institute of Pathology, Rockville, MD
Short tandem repeats (STRs) are highly polymorphic regions of DNA dispersed throughout the human genome. Analysis of multiple STR loci provides a powerful technique for identification of human remains. Amplification of multiplexed STRs, combined with automated fluorescent detection, offers an extremely sensitive and cost-effective method for rapid analysis of biological specimens in routine casework.
The purpose of this study was to validate the ABI PRISM™ STR Primer Set for use in a multiplex system according to TWGDAM guidelines. The ABI PRISM™ STR Primer Set consists of fluorescently labeled primer pairs which direct the amplification of the following four loci: vWA31, TH01, F13A1 FES/FPS. Validation studies included the determination of the minimum sample required to obtain a typeable result, reproducibility of samples, consistency and precision of results, non-probative case samples and the comparison to previously utilized primer sets for these STR loci (Forensic Science Service, Birmingham, England). The results of this study will be presented.
The opinions and assertions expressed herein are solely those of the authors and are not to be construed as official or as the views of the United States Department of Defense or the United States Department of the Army.
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