Rosane Silva, PhD, Elizeu F. De Carvalho, PhD and Rodrigo S. Moura-Neto, PhD
Genealogica Diagnosticos Moleculares, Rua Voluntario da Patri, 445/506, Rio de Janeiro,
Brazil
All paternity cases were performed at the Laboratory Genealogica, in Rio de Janeiro, Brazil. The samples were analyzed using a combination of seven VNTR probes chosen among the following: D1S7 (MS1), D2S44 (YNH24), D4S139 (PH30), D5S110 (LH1), D6S132 (AC424), D7S467 (AC415), D10S28 (TBQ7), D14S13 (CMM101), D17S26 (EFD52). DNA obtained from samples of peripheral blood were digested with Hae III, separated in 0.8% agarose gels, transferred and hybridized to alkaline phosphatase-conjugated probes. Allele sizings were performed using the Gibco Ladder as molecular weight marker. We have observed that 79% of the cases were inclusions and 21% exclusions. We have compared fixed bin frequency estimation from six loci obtained from non-related alleged father and mother with the VNTR Population Data: Worldwide Study (Federal Bureau of Investigation, U.S. Department of Justice, 1993).
The table below represents the data obtained for six loci.
D1S7 |
D2S44 |
D4S139 |
D5S110 |
D10S28 |
D17S26 |
|
| N | 298 | 268 | 302 | 258 | 293 | 390 |
| H | 5.5 | 7.2 | 9.6 | 5.7 | 5.9 | 6.5 |
| h | 94.5 | 92.8 | 90.4 | 94.3 | 94.1 | 93.5 |
| PI | 9.0 | 6.9 | 5.2 | 8.8 | 8.4 | 7.7 |
| PE | 89.6 | 85.7 | 81.0 | 88.7 | 88.3 | 87.1 |
N = number of chromosomes; H = homozygous; h = heterozygous; PI = paternity index; PE = power of exclusion.
Three band patterns were observed at loci D4S139 (0.0058), D5S110 (0.0058), D7S467 (0.02). Mutation rate was higher for D5S110 (0.023), D4S139 (0.015), D10S28 (0.008) and D14S13 (0.045).
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