F. Al-Shamali1, A. Al-Khayat1 and B. Budowle2
1Dubai Police, Crime Laboratory, CID, Dubai, United Arab Emirates
2Forensic Science Research and Training Center, Laboratory Division, FBI
Academy, Quantico, VA
A special class of variable number of tandem repeats (VNTR) that could prove useful for forensic identity testing are the AMP-FLP markers. One such highly polymorphic system widely characterized by forensic scientists is the D1S80 locus.
Population data are necessary to convey an estimate of significance of a DNA match. In this study, gene frequencies for the D1S80 locus are determined in a Dubaian Arab population of 93 unrelated individuals. Amplification was carried out in a 9600 Gene Amp PCR system, using the AmpliFLP™ D1S80 PCR Amplification kit (Roche Molecular Systems, Inc.) followed by separation on polyacrylamide gels and silver staining. Alleles were called out by comparison to an allelic ladder. The data were subsequently subjected to multiple statistical tests. 15 alleles were observed, the highest frequency being 0.269 for allele 18 and 0.419 for allele 24. The observed homozygosity index is 30.1 percent. The sample population meet Hardy-Weinberg expectation (HWE). The frequency data can be used in both forensic and paternity testing.
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