U. Schwab1, F. Chernomas2, L. Larcom2 and J. Weems1
1Clemson University/Greenville Hospital Systems Biomedical Cooperative
2Department of Physics, Clemson University
Torulopsis glabrata (Candida glabrata), an opportunistic pathogen, has been seen increasingly as a nosocomial pathogen. Because of the lack of a reliable system for detecting differences within the same species, little is known about the epidemiology of infection with T. glabrata. We evaluated the reproducibility and discriminatory power of random amplified polymorphic DNA (RAPD) analysis of T.glabrata as compared to electrophoretic karyotyping (EK) using contour clamped homogeneous field electrophoresis. Forty-eight isolates from 37 patients were evaluated. EK and RAPD were performed on each of these isolates, and the results of the two procedures were compared. The EK method revealed 19 different types of T. Glabrata, whereas the RAPD analysis for estimation of genetic relationships among T. Glabrata appears to have some advantages over EK, the number of distinct DNA types observed is fewer when compared to EK.
Go to proceedings home page