Debbie A. Kennedy and Colleen K. Proffitt
Arizona DPS Crime Laboratory, Phoenix, AZ
Amplification of DNA in forensic cases using the Polymerase Chain Reaction (PCR) can provide useful information when biological evidence is too small or too degraded for Restriction Fragment Length Polymorphism (RFLP) analysis. The HLA-DQA1 and D1S80 loci are two of the PCR-based genetic markers that have been validated for forensic use. Database information is available for these systems for the major racial and ethnic populations. However, since there are many Native Americans in Arizona, a database for these populations was needed.
Navajo and Apache blood samples previously collected for the establishment of the Arizona RFLP database were extracted with Chelex, then amplified and typed using the HLA-DQa and D1S80 kits from Perkin-Elmer. The observed alleles were tabulated and the allele frequencies were calculated. The results of these studies will be presented.
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