A.S. Greef, C.G. Clay and B.E de Villiers
Section Molecular Biology and Diagnostics, Medical University of Southern Africa, South
Africa
Due to differences in the frequency of distribution of particular STR allelic markers reported for different ethnic groups' databases on allelic frequency distribution in different ethnic groups are required to estimate the power of exclusion of different combinations of STR allelic markers. We studied the frequency distribution of 70 discrete STR allelic markers located on 8 different chromosomes in the South African Black (n=240) and Caucasian (n=200) population groups.
DNA was extracted from whole blood by a modified Chelex method. Mono and multiplex amplified DNA was sized on PAGE gels using discrete allelic markers. The frequency distribution of sized markers was scored and compared between the two population groups. Although the frequency distribution of the majority of STR allelic markers were similar significant differences were seen in some markers, notably the following: FES (8) Black 16.7%, Caucasian 1.9%; CSF1PO (12) Black 38.8%, Caucasian 21.3%; TPOX (8) Black 27.8%, Caucasian 46.3%; TH01 (10) Black 1.5%, Caucasian 34.1%; TH01 (7) Black 39.7%, Caucasian 13.6%. Putative new/rare alleles were occasionally seen: vWF at position 12, TPOX at 6 and 7, CSF1PO at 6 and F13AO1 at position 3.
We have successfully applied this technology to degraded tissue from cadavers, semen, vaginal swabs and blood stains scraped from a variety of surfaces to establish human identity/paternity.
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