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Evaluation of a New STR Multiplex (D5S818, D13S317, D7S820) for Forensic Applications

R.M. Fourney¹, J.C. Elliott¹, M. Buoncristiani², J.M. Robertson,³ K.L. Bowen¹, B. Leclair¹ and G. R. Carmody^1,4
1Biology Services, Central Forensic Laboratory, Royal Canadian Mounted Police, Ottawa, Ontario, Canada
²California Department of Justice DNA Laboratory, Berkeley, CA
³Applied Biosystems Division, Perkin-Elmer, Foster City, CA
⁴Dept. of Biology, Carleton University, Ottawa, Ontario, Canada

In recent years both the forensic and legal communities have grown accustomed to elevated levels of discrimination using RFLP VNTR analysis. In order for PCR-based DNA identification to achieve similar genotype discrimination, the identification and evaluation of additional STR systems will be required. This report describes a new STR triplex composed of three tetranucleotide repeat loci (D5S818, D7S820, and D13S317) that has a combined potential discrimination of 0.999 as calculated using a Canadian Caucasian database. In addition, no new alleles were discovered in the preliminary evaluation of Canadian Aboriginal samples. Automated fluorescent allele detection with DNA sequencers (Applied Biosystems Division-Perkin Elmer) were used to assign allele designations, investigate precision (e.g. with internal lane molecular weight standards) and test the robustness and forensic efficacy of this multiplex. In general, both amplification studies using a dilution series of human genomic DNA standards as well as forensic test samples demonstrate the reliability and sensitivity of this triplex. More detailed results are presented in tabular and graphical form.

Characteristics of this tetranucleotide triplex are noted below:

PD: Probability of discrimination between individuals, *allele size in box

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