|
AmpliFLP™ D1S80 Validation and Population Study for IndianaT.W. Bille, K.S. Epperson, C.A. Sobieralski, J.M. Steinbruck and S.F. Mayer In order to further increase the power of discrimination for DNA analysis performed at the Indiana State Police Laboratory, an additional marker, D1S80, has been examined for future casework implementation. The markers used currently, HLA DQA1 and the PM system, are sequence polymorphisms which utilize the Polymerase Chain Reaction to amplify a specific region of the genomic DNA. In contrast, the D1S80 locus is a length polymorphism which also utilizes the Polymerase Chain Reaction. The alleles of this variable number tandem repeat (VNTR) consist of 16 base pair core units repeated between 14 and 41 times, with a few exceptions. These alleles can then be separated by PAGE electrophoresis and silver stained for visualization. Caucasian and Black population data bases were compiled, analyzed and are currently being tested for Hardy-Weinberg equilibrium. Two extraction protocols, Chelex and phenol/chloroform isoamyl alcohol with microcon concentration, were compared. Blood, semen and saliva stains along with pulled hairs were analyzed. The effects of exposing bloodstains to environmental insults and chemical contaminants such as fingerprint powder, gun oil, soil, Takayama solution, Luminol solution, ultraviolet light and laser light, were studied. Bloodstains on ten different substrates were tested. Minimum samples size, dilution, reproducibility and mixture studies were performed. Analysis was attempted on bloodstains from several different common animals. The addition of bovine serum albumin (BSA) was examined to determine the effects on the amplification process. The results of this study confirm the reliability of the AmpliFLP™ D1S80 PCR Amplification and Typing Kit. Go to proceedings home page
|
|