Promega PubHub Articles

Full-size Humor

Tue, 10 Apr 2012 09:22:14 -0500

FAQ for RT-qPCR

Fri, 06 Apr 2012 14:44:13 -0500

This article is a serries of questions and answers about RT-qPCR. It includes a glossery of terms and links to realated resources.

Getting the Best Results from Your Reverse Transcription Quantitative PCR Assay

Fri, 06 Apr 2012 10:26:59 -0500

This article describes the key considerations for reverse transcription (RT) qPCR.

Cell-Free Expression Systems to Study HDAC Protein Function

Tue, 27 Mar 2012 14:28:27 -0500

This article describes cell-free expression and capture of histone deacetylase (HDAC) proteins using the TnT® T7 Insect Cell Extract System, HaloTag® labeling and Maxwell® 16 isolation technologies.

Supplemental Information for RNA Quantification Using the GoTaq® RT-qPCR Systems

Mon, 26 Mar 2012 13:55:37 -0500

Information to accompany the PubHub article "RNA Quantification Using the GoTaq RT-qPCR Systems"

RNA Quantification Using the GoTaq® RT-qPCR Systems

Mon, 26 Mar 2012 13:18:22 -0500

This article demonstrates how well the GoTaq 1-Step and 2-Step RT-qPCR Systems perform and compare to four competitor one-step RT-qPCR systems.

Review: Interaction of 3ß-Hydroxysteroid Dehydrogenase with Mitochondrial Translocases

Wed, 14 Mar 2012 09:42:39 -0500

A paper describing use of the TNT® Reticulocyte Lysate System to synthesize substrates for mitochondrial import assays.

Review: Examining Complexity of Human RNA Polymerases Using HaloTag® Technology Coupled to Label Free Quantitative Proteomics

Tue, 13 Mar 2012 14:49:09 -0500

Review of an article published in the Journal of Proteome Research. The HaloTag Technology was used to examine RNA polymerases and their complexes. Techniques included protein pull-downs of unique interactors and cellular localization of the associated complexes.

Monitor Cell Viability and Reporter Gene Expression in the Same Well with the One-Glo™+Tox Luciferase Reporter and Cell Viability Assay

Wed, 15 Feb 2012 12:38:11 -0600

A brief article describing how a single-well assay can aid in interpretation of reporter assay data.

Reverse Transfection Using FuGENE® 6 and FuGENE® HD

Mon, 13 Feb 2012 11:56:57 -0600

In this article, we tested the ability of FuGENE® 6 and FuGENE® HD to “reverse” transfect three cell lines and monitored efficiency and toxicity using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay.

A Hat Tip to the DNA-Mediated Heritable Transformation of a Biochemical Trait on Its 50-Year Anniversary

Wed, 08 Feb 2012 10:45:42 -0600

Here we review the 1962 paper of Szybalska and Szybalski describing the development and use of HAT medium and the first described DNA-mediated heritable transformation of a biochemical trait in mammalian cells.

Add-mix-read assays for assessing cell health using an iPS cardiomyocyte cell model

Mon, 06 Feb 2012 12:46:33 -0600

Here we present data generated with add-mix-read cell health assays and compare results obtained using K562 cells and iPS cardiomyocytes for prediciting valid off-target effects of therapeutic agents.

Full-size Humor

Tue, 31 Jan 2012 08:31:48 -0600

GoTaq® Long PCR Master Mix for Reliable Amplification of Long PCR Targets

Mon, 14 Nov 2011 11:25:26 -0600

This article describes the features and benefits of the GoTaq® Long PCR Master Mix. The Master Mix combines the proven performance of the GoTaq® Hot Start Polymerase with the higher fidelity of a proofreading polymerase.

Effective Determination of Cellular Viability in Microtissue Spheroids

Thu, 03 Nov 2011 13:19:19 -0500

This article describes ATP as a definitive biomarker of cell viability using the CellTiter-Glo Luminescent Cell Viability Assay and microtissue spheroids. Important cell-to-cell interactions can be recreated in vitro by 3-dimensional cell culture models.