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There are 6 pages to this Feature:
Molecular Approaches to Neuroscience
- The Main Course - A Satisfied Customer - Q&A - BTC - Photos
& References
Photos & References
Lih-Fen Lue, Sun Health Research Institute, loading an
electrophoresis gel.
Lih-Fen Lue (left) and Laura Scott, Amgen (right),
are pleased with the results from their PCR product cloning experiment.
Jeff Kramer and Ed Plowey, University of Illinois (right).
Kyle Hooper, course instructor and Promega Technical Services
Scientist, speaks with Kristi Buxton about the Northern blotting procedures.
Terri Sundquist, course instructor and Promega Technical Services
Scientist, proudly displays the results of the Northern blots (of 18s RNA and G3PDH) to
the class. We got signal!
Gaylen Edwards, University of Georgia, and Mary Haak-Frendscho,
Instructor and Promega scientist.
Laura Scott, Amgen, applies the hand-warming technique to her
sample.
Fernando Cardozo-Pelaez, University of South Florida (left)
and Brian Pike, University of Texas-Houston (right).
Kristi Buxton, Battelle Memorial Institute.
Anne Ratashak, Nebraska Medical Center (left), and
Lynda Wright, University of Wisconsin (right), prepare their RNA samples.
References
- Cao, Y.Q. et al. (1998) Primary afferent tachykinins are
required to experience moderate to intense pain. Nature 392,
390.
Recommended References by Subject
Molecular Biology
Protocols and
Applications Guide, Third Edition, Promega Corporation.
Current Protocols in Molecular Biology, Ausubel, F.M. et al.,
eds.,Wiley & Sons, NY.
Molecular Cloning: A Laboratory Manual, (1989) Second Edition, Sambrook, J.,
Fritsch, E.F. and Maniatis, T., eds., Cold Spring Harbor Laboratory Press, Cold Spring
Harbor, NY.
LabFax:
Molecular Biology Second Edition Vol. I, Recombinant DNA, (1998) Brown, T.A., ed., Academic Press, NY.
LabFax: Molecular
Biology Second Edition Vol. II, Gene Anaylsis, (1998) Brown, T.A., ed., Academic
Press, NY.
Neuroscience
Current Protocols in Neuroscience, (1997) Crawley, J. et al., eds.,
John Wiley & Sons, Ltd.
Neuroscience
LabFax, (1997) Lynch, M.A. and O'Mara, S.M., eds., Academic Press, NY.
Experimental
Neuroanatomy: A Practical Approach, (1992), Bolam, J.P., Oxford University Press,
Inc.
Neural Cell
Culture: A Practical Approach, (1996) Cohen, J. and Wilkin,
G.P., Oxford University Press, Inc.
Immunology
Current Protocols in Immunology, (1992) Coligan, J.E. et al.,
eds.,Wiley & Sons, NY.
Molecular
Immunology, Second Edition, (1996) Hames, B.D. and Glover, D.M., eds., Oxford
University Press, Inc.
Amplification
PCR1: A Practical
Approach, (1991; reprinted 1994) McPherson, M.J., Taylor, G.R. and Quirke, P.,
eds., Oxford University Press, Inc.
PCR2: A Practical
Approach, (1995) McPherson, M.J., Hames, B.D. and Taylor, G.R., eds., Oxford
University Press, Inc.
PCR3: A Practical
Approach, (1998) Herrington, C.S. and O'Leary, J.J., eds., Oxford University
Press, Inc.
PCR (Introduction
to Biotechniques), Second Edition, (1997) Newton, C.R. and Graham, A., eds.,
Spriner Verlag.
PCR
Cloning Protocols: from Molecular Cloning to Genetic Engineering, (1996) White, B.A., ed., Humana Press.
PCR: Essential
Techniques, (1996) Burke, J.F., ed., John Wiley & Sons, Ltd.
RNA
RNA Methodologies: A Laboratory Guide for Isolation and Characterization,
Second Edition, Farrell, R.E., Jr., ed., Academic Press, NY.
Acknowledgments
The author thanks Allan I. Basbaum for contributing to this story. Dr.
Basbaum is in the Department of Anatomy, W.M. Keck Foundation Center for Integrative
Neuroscience, and Department of Pediatrics, University of California, San Francisco, and
is a contributing editor to the Journal of Neuroscience.
(a)Patent Pending.
(b)The PCR process is covered by patents issued and applicable in
certain countries. Promega does not encourage or support the unauthorized or unlicensed
use of the PCR process. Use of this product is recommended for persons that either have a
license to perform PCR or are not required to obtain a license.
(c)U.S. Pat. No. 4,766,072.
(d)Licensed under one or both of U.S. Pat. No. 5,487,993 and European
Pat. No. 0 550 693.
pGEM is a registered trademark of Promega Corporation.
GenBank is a registered trademark of the U.S. Department of Health and
Human Services.
All photos by Neal Cosby and property of Promega Corporation.
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