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There are 6 pages to this Feature:
Molecular Approaches to Neuroscience
- The Main Course - A Satisfied
Customer - Q&A - BTC - Photos & References
The Main Course: Molecular Approaches to Neuroscience
The hands-on laboratory and lecture course, Molecular Approaches to
Neuroscience, offers classically trained neuroscientists experience measuring gene
expression in relevant tissues. Promega scientists cover many molecular techniques with
applications in neuroscience research. Specific topics include: RNA purification, RNase
protection assays, PCR(b), RT-PCR and quantitative
RT-PCR, T-vector cloning, Northern blotting, transcription reactions, and Western blotting
and ELISAs.
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Each entree includes a complimentary
(and complementary!) laboratory exercise. |
- RNA: The current methodologies used to isolate and purify total cellular RNA and
poly(A)+ RNA.
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- Northern Blot Analysis: Includes complete theoretical and practical
considerations of agarose gel electrophoresis systems, membrane types, Northern blot
transfer techniques and hybridization parameters.
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- RNase Protection Assay: How to analyze RNA. Includes template and probe
preparation, solution hybridization, RNase digestion and polyacrylamide gel analysis of
products.
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- PCR Introduction: PCR and cloning of
PCR products using the pGEM®-T Easy
Vector(c,d), a T-tailed cloning vector.
Introduction to reverse transcriptase-PCR (RT-PCR) as a means of amplifying specific
target RNA from either total cellular or poly(A)+ RNA fractions. Introduction to
RT-PCR as a quantitative technique.
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- Western Blot and ELISA: How to analyze proteins. Includes
polyacrylamide gel electrophoresis systems, membrane types, Western blotting, detection
strategies and soluble immunoassay (ELISA)
strategies.
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The next Molecular Approaches to Neuroscience course
is June 11-15, 2001. Contact Rick Salatino
(608-274-4300; FAX: 608-277-2516) for additional course content information, or
register online. |
Rapid PCR product ligation using the pGEM®-T Easy
Vector System and the new 2X Rapid Ligation Buffer.
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