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Molecular Approaches to Neuroscience - The Main Course - A Satisfied Customer - Q&A - BTC - Photos & References

Photos & References 

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Lih-Fen Lue, Sun Health Research Institute, loading an electrophoresis gel.

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Lih-Fen Lue (left) and Laura Scott, Amgen (right), are pleased with the results from their PCR product cloning experiment.

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Jeff Kramer and Ed Plowey, University of Illinois (right).

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Kyle Hooper, course instructor and Promega Technical Services Scientist, speaks with Kristi Buxton about the Northern blotting procedures.

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Terri Sundquist, course instructor and Promega Technical Services Scientist, proudly displays the results of the Northern blots (of 18s RNA and G3PDH) to the class. We got signal!

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Gaylen Edwards, University of Georgia, and Mary Haak-Frendscho, Instructor and Promega scientist.

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Laura Scott, Amgen, applies the hand-warming technique to her sample.

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Fernando Cardozo-Pelaez, University of South Florida (left) and Brian Pike, University of Texas-Houston (right).

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Kristi Buxton, Battelle Memorial Institute.

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Anne Ratashak, Nebraska Medical Center (left), and Lynda Wright, University of Wisconsin (right), prepare their RNA samples.

References

  1. Cao, Y.Q. et al. (1998) Primary afferent tachykinins are required to experience moderate to intense pain. Nature 392, 390.

Recommended References by Subject

Molecular Biology

Protocols and Applications Guide, Third Edition, Promega Corporation.

Current Protocols in Molecular Biology, Ausubel, F.M. et al., eds.,Wiley & Sons, NY.

Molecular Cloning: A Laboratory Manual, (1989) Second Edition, Sambrook, J., Fritsch, E.F. and Maniatis, T., eds., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

LabFax: Molecular Biology Second Edition Vol. I, Recombinant DNA, (1998) Brown, T.A., ed., Academic Press, NY.

LabFax: Molecular Biology Second Edition Vol. II, Gene Anaylsis, (1998) Brown, T.A., ed., Academic Press, NY.

Neuroscience

Current Protocols in Neuroscience, (1997) Crawley, J. et al., eds., John Wiley & Sons, Ltd.

Neuroscience LabFax, (1997) Lynch, M.A. and O'Mara, S.M., eds., Academic Press, NY. 

Experimental Neuroanatomy: A Practical Approach, (1992), Bolam, J.P., Oxford University Press, Inc.

Neural Cell Culture: A Practical Approach,  (1996) Cohen, J. and Wilkin, G.P., Oxford University Press, Inc.

Immunology

Current Protocols in Immunology, (1992) Coligan, J.E. et al., eds.,Wiley & Sons, NY.

Molecular Immunology, Second Edition, (1996) Hames, B.D. and Glover, D.M., eds., Oxford University Press, Inc.

Amplification

PCR1: A Practical Approach, (1991; reprinted 1994) McPherson, M.J., Taylor, G.R. and Quirke, P., eds., Oxford University Press, Inc.

PCR2: A Practical Approach, (1995) McPherson, M.J., Hames, B.D. and Taylor, G.R., eds., Oxford University Press, Inc.

PCR3: A Practical Approach, (1998) Herrington, C.S. and O'Leary, J.J., eds., Oxford University Press, Inc.

PCR (Introduction to Biotechniques), Second Edition, (1997) Newton, C.R. and Graham, A., eds., Spriner Verlag.

PCR Cloning Protocols: from Molecular Cloning to Genetic Engineering, (1996) White, B.A., ed., Humana Press.

PCR: Essential Techniques, (1996) Burke, J.F., ed., John Wiley & Sons, Ltd.

RNA

RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Second Edition, Farrell, R.E., Jr., ed., Academic Press, NY.

Acknowledgments

The author thanks Allan I. Basbaum for contributing to this story. Dr. Basbaum is in the Department of Anatomy, W.M. Keck Foundation Center for Integrative Neuroscience, and Department of Pediatrics, University of California, San Francisco, and is a contributing editor to the Journal of Neuroscience.

(a)Patent Pending.

(b)The PCR process is covered by patents issued and applicable in certain countries. Promega does not encourage or support the unauthorized or unlicensed use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not required to obtain a license.

(c)U.S. Pat. No. 4,766,072.

(d)Licensed under one or both of U.S. Pat. No. 5,487,993 and European Pat. No. 0 550 693.

pGEM is a registered trademark of Promega Corporation.

GenBank is a registered trademark of the U.S. Department of Health and Human Services.

All photos by Neal Cosby and property of Promega Corporation.