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Reagents & Solutions 800-356-9526 608-274-4330 enotes@promega.com
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FAQspeakCan I control the addition of nucleotides to the 3´ end of DNA while using terminal deoxynucleotidyl transferase (TdT)?TdT catalyzes the template-independent addition of dNTPs to the 3´ hydroxyl termini of DNA. Single-stranded DNA molecules are the preferred substrate for TdT, but double-stranded DNA molecules with 3´-hydroxyl termini can also be used when Co2+ is used in the reaction mixture. In the presence of Co2+ ions, TdT will preferentially add pyrimidine nucleotides (dCTP and dTTP) to the end of a DNA molecule. In the presence of Mg2+ ions, TdT prefers to add purine nucleotides (dATP and dGTP). The number of nucleotides added to each molecule will be determined by the molar ratio of nucleotides to DNA molecules. For example, in the presence of 10pmol of nucleotides and 1pmol DNA, the average tail will be 10 nucleotides in length. Products may be covered by pending or issued patents or may have certain limitations on use. Please visit our patent and trademark web page for more information. |
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