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Reagents & Solutions 800-356-9526 608-274-4330 enotes@promega.com
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FAQspeakWhich DNA polymerase is preferred for removal of a 3´ DNA overhang or for filling in a 5´ overhang?While both T4 DNA Polymerase (Cat.# M4211) and DNA Polymerase I Large (Klenow) Fragment (Cat.# M2201) have a 3´→ 5´ exonuclease activity that can be used to enzymatically remove a 3´ overhang, T4 DNA Polymerase is often the preferred enzyme. T4 DNA Polymerase has a stronger 3´→ 5´ exonuclease activity that will remove the overhang more efficiently. Both enzymes can be used to fill in a 5´ overhang, and there is not often a preference between these enzymes. The removal of a 3´ overhang with T4 DNA Polymerase requires the addition of dNTPs. *Products may be covered by pending or issued patents. Please visit our patent and trademark web page for more information. |
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