 |
FAQspeakHow do you use the FITC-VAD-FMK In Situ Marker of apoptosis to label cells undergoing apoptosis?The CaspACE™ FITC-VAD-FMK In Situ Marker (Cat.# G7461, G7462) is a tool that allows for the most rapid means of detecting active caspases within cells without actually lysing the cells. FITC-VAD-FMK is a fluorescent derivative of the cell-permeable pan caspase inhibitor, Z-VAD-FMK. FITC-VAD-FMK crosses the cell membrane, irreversibly binds activated caspases, labels them with a fluorescent tag and inactivates those caspases. Basically, cells are treated with an apoptosis-inducing agent. After a period of time, FITC-VAD-FMK is added and reacted with the cells for 20 minutes. The cells are then washed and analyzed. To preserve the signal, the cells may be fixed after washing away any unbound FITC-VAD-FMK. If using a fluorescent microscope, analysis of a sample from start to finish can be performed in <30 minutes. FITC-VAD-FMK should not be used on fixed cells or tissues, as the caspases generally do not retain activity after fixing. To read more about CaspACE™ FITC-VAD-FMK In Situ Marker, see references 1–4. References
*Products may be covered by pending or issued patents. Please visit our patent and trademark web page for more information.CaspACE is a trademark of Promega Corporation. |
|||||
|
||||||
