By Terri Grunst, B.S.
Promega Corporation

• Wizard^® Genomic DNA Purification Kit and the Isolation of Plant Genomic DNA

  By Sonja Vorwerk, B.S.
  Max Planck Institut for Plant Breeding Research, Cologne, Germany

• A Simple and Reproducible Method to Isolate Plasmid DNA from Yeast after a Two-Hybrid Screening 

By Gaël Nicolas, Ph.D., Catherine Fournier, Ph.D., Colette Galand, B.S., and Marie-Christine Lecomte, Ph.D.
Faculty of Medicine, Xavier Bichat, France

• Isolation of Genomic DNA from Small Volumes of Whole Blood Using the Wizard^® Genomic DNA Purification Kit

By Natalie Betz, Ph.D.
Promega Corporation

• Specific Capture of Biotinylated Nucleic Acids on the SAM^2® Biotin Capture Membrane

By Daniel Kephart, Ph.D.
Promega Corporation

• Extraction and Amplification of DNA from an Ancient Moss

By Damien Marsic¹, M.S., Richard Hoover², Ph.D., and Joe D. Ng¹, Ph.D.
¹University of Alabama in Huntsville, and ²Marshall Space Flight Center, Huntsville, AL

gene expression & reporters

• Using the GloMax^®-Multi Detection System for Measuring Signal from Multiplexed Fluorescent and Luminescent Assays

  By Halina Zakowicz, Ph.D., Michael Bjerke, M.S. and Trista Schagat, Ph.D.
  Promega Corporation

• Using the Beta-Glo^® Assay System to Demonstrate Transport of β-Galactosidase Across the Blood-Brain Barrier

  From the article: Zhang, Y. and Pardridge, W.M. (2005) Delivery of β-Galactosidase to Mouse Brain via the Blood-Brain Barrier Transferrin Receptor. J. Pharmacol. Exp. Ther. 313, 1075–81.

• Sensitive Homogeneous CEDIA^® Analysis Using the Beta-Glo^® Assay System

  From the article: Yang, X., Janatova, J. and Andrade, J.D. (2005) Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors. Anal. Biochem. 336, 102–7. Department of Bioengineering, University of Utah, 50 South Central Campus Drive, Room 2480, Salt Lake City, UT 84112-9202, USA.

• Quantitating Protein in Promega Lysis Buffers and Steady-Glo^® and Bright-Glo™ Luciferase Reagents

By Natalie Betz, Ph.D.
Promega Corporation

• Protein Determination in Promega Cell Lysis Buffers Using a Noninterfering Protein Assay

  By Natalie Betz, Ph.D.
  Promega Corporation

• Alpha-Melanocyte-Stimulating Hormone Induction of Luciferase Expression Under the Control of Human Tyrosinase Promoter/Enhancer

By Vassili Zakhartchenko¹, M.S., and Yanina Rozenberg², Ph.D.
¹Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry and ²Targeted Artificial Gene Delivery Group, Gene Therapy Laboratories, University of Southern California Keck School of Medicine, Los Angeles, CA

• Compatibility of the CellTiter 96^® AQueous One Solution with the Luciferase Assay System

By Natalie Betz, Ph.D.
Promega Corporation

immunological detection

• AttoPhos^® AP Fluorescent Substrate and Western Blue^® Stabilized Substrate Detect Cytoplasmic Alkaline Phosphatase in NIH3T3 Cells  

  By Randy Hoffman, B.S., and Lisa DeBerg, M.S.
  Promega Corporation

• Specific Labeling of Neurons and Glia in Mixed Cerebrocortical Cultures

By Thomas Gillessen, Franz Tokai and Kati Breiter, Institut fuer Pharmakologie und Toxikologie, Sanitaetsakademie der Bundeswehr, Germany

in vitro transcription

• T7 RiboMAX™ Express: Generation of 27kb in vitro Transcripts in Minutes

  By Barbara Schelle and Volker Thiel, Ph.D. 
  Institute of Virology and Immunology, University of Würzburg, 97078 Würzburg, Germany

• A Simple and Efficient Method to Produce RNAs with Homogenous 3´ Ends  

  By Cheng Kao, Ph.D.¹,  S. Rüdisser², Ph.D., and M. Zheng², Ph.D.
  ¹Department of Biology, Indiana University, Bloomington, IN 47405 USA; ²Department of Chemistry, University of California, Berkeley, and Lawrence Berkeley National Laboratory, Berkeley, CA 97420-1460 USA

• Use of MagneHis™ Particles and His-Tagged RNA Polymerase to Study Stalled Elongation Complexes at DNA Lesions 

  From the article Jung, Y. and Lippard, S.J. (2003) J. Biol. Chem. 278, 52084–92.
  Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA

microarrays

• The ChipShot™ Membrane Clean-Up System: A Fast Simple Technique for Efficient Recovery of Highly Purified cDNA

  By Nadine Nassif, M.S.
  Promega Corporation

• Microarray Applications Using Promega Products

By Randy Hoffman, B.S.
Promega Corporation

• Using ImProm-II™ Reverse Transcription System to Generate Fluorescent Probes

  By Natalie Betz, Ph.D., Randy Hoffman, B.S., Bing Hui Lin and Katharine Miller
  Promega Corporation

• Isolating High-Quality mRNA From Total RNA with the PolyATtract^® mRNA Isolation System For Use in Expression Profiling with the Pronto!™ Plus System

  By Patricia Fulmer, Ph.D., Dawn Rabbach, A.A.S., and Nadine Nassif, M.S.
  Promega Corporation

pcr

• Activity of Promega Restriction Enzymes in GoTaq^® Green Master Mix and PCR Master Mix

  By David Tritle, M.S.
  Promega Corporation

• Analyses of gene disruption by whole-cell PCR using the GoTaq^® Green Master Mix

  By Srinivas Venkatram, Ph.D
  Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232

• Compatibility of GoTaq™ DNA Polymerase-Amplified PCR Products with TnT^® T7 Quick for PCR DNA

  By Natalie Betz, Ph.D.
  Promega Corporation

• Advantages Designed into Promega's PCR Master Mix

  By Mark Denhart, M.S. and Vinayak Doraiswamy, Ph.D.
  Promega Corporation

• Characterization of TNT^® T7 Quick for PCR DNA

  By Natalie Betz, Ph.D.
  Promega Corporation

• Long PCR Amplification of p300 cDNA Using a Blend of Taq DNA Polymerase and Proofreading Pfu DNA Polymerase

By Joost Martens
MCB1, Leiden University Medical Center, Wassenaarseweg 72, Leiden, The Netherlands

• Screening of the Gamµ-7 Microsatellite Locus to Determine the Sex of Captive Whooping Cranes

By Kenneth L. Jones, M.S.¹, and Travis C. Glenn, Ph.D.^2
1Texas A&M University-Kingsville and ²Savannah River Ecology Laboratory

• Extraction and Amplification of DNA from an Ancient Moss

By Damien Marsic¹, M.S., Richard Hoover², Ph.D., and Joe D. Ng¹, Ph.D.
¹University of Alabama in Huntsville, and ²Marshall Space Flight Center, Huntsville, AL

 plant biotechnology

• Isolation of Genomic DNA from Agricultural Bacteria Using the Wizard^® MagneSil™ Plasmid Purification System

  By Abigail Farfan, M.A., Hanan Abramovici, M.S., and Terri Grunst, B.S.
  Promega Corporation

• Wizard^® Genomic DNA Purification Kit and the Isolation of Plant Genomic DNA

  By Sonja Vorwerk, B.S.
  Max Planck Institut for Plant Breeding Research, Cologne, Germany

• Titration of Tomato Leaf Material Used as Starting Material for SV Total RNA Isolation

By Hemanth Shenoi, Ph.D., and Daniel Kephart, Ph.D.
Promega Corporation

• Automated Isolation of Genomic DNA Using Promega’s Wizard^® SV 96 DNA Binding Plates on the Beckman Biomek^® 2000

By Terri Grunst, B.S.
Promega Corporation

• Automated Poly(A)+ mRNA Isolation From Plant Tissues Using the PolyATtract^® System 1000

By Tracy Worzella, B.S. and Terri Grunst, B.S.
Promega Corporation

 protein:nucleic acid interactions

• Using the Beta-Glo^® Assay System to Determine β-galactosidase Activity in Yeast: Applications for Yeast Two- and Three-Hybrid Systems

  By Brad Hook, Ph.D., David Bernstein, Ph.D. and Marvin Wickens, Ph.D.
  Department of Biochemistry, University of Wisconsin, Madison, WI 53706

• Using SAM^2® Biotin Capture Membranes to Study Protein:DNA Binding

  By Natalie Betz, Ph.D.
  Promega Corporation

• Non-Radioactive Gel Shift Assays

  By Natalie Betz, Ph.D.
  Promega Corporation

• pGEM^®-T Easy Vector System is an Easy Tool for Preparing Gel Shift Probes

  By Huai Wang and Sharyn E. Perry
  Department of Agronomy, University of Kentucky, Lexington, KY 40546

• Use of MagneHis™ Particles and His-Tagged RNA Polymerase to Study Stalled Elongation Complexes at DNA Lesions 

  From the article Jung, Y. and Lippard, S.J. (2003) J. Biol. Chem. 278, 52084–92.
  Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA

protein expression & analysis

• ProTEV Protease for Fusion Protein Processing

  By Jessica Anderson, Ph.D., Jami English, M.S., and John Shultz, Ph.D.
  Promega Corporation

• Protein Expression in Less Time: A Short Induction Protocol for KRX

  By Lynn Litterer, Ph.D., and Trista Schagat, Ph.D.,
  Promega Corporation

• Non-Radioactive Identification of Protein:Protein Interactions Using TNT^® SP6 High-Yield Protein Expression System

  By Trista Schagat, Ph.D., Natalie Betz, Ph.D. and Jacqui Mendez, M.S.
  Promega Corporation

• Use of anti-β-galactosidase antibody to identify transplanted bone marrow-derived cells expressing bacterial β-galactosidase

  From the article: Houghton, J. et al.. (2004) Science 306, 1568–71.
  Department of Medicine and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.

• Alternative Elution Conditions for the Mass Spectrometry Analysis of Polyhistidine-Tagged Proteins

  By Laurie Engel, B.S., Becky Godat, M.A., Rod Flemming, Ph.D., and Tonny Johnson, Ph.D.
  Promega Corporation

• Dephosphorylation of Proteins with Shrimp Alkaline Phosphatase

  From the article Jonas, B.A. and Privalsky, M.L. (2004) J. Biol. Chem. 279, 54676–86.
  Section of Microbiology, Division of Biological Sciences, University of California, Davis, California 95616

• Purification of Recombinant Polyhistidine-Tagged Proteins From Mammalian Cells Using the MagneHis™ Protein Purification System

  By Natalie Betz, Ph.D.
  Promega Corporation

• Effects of Various Additives or Contaminants on in vitro Transcription/Translation in the Gold TNT® T7 Express 96 System

  By Natalie Betz, Ph.D.
  Promega Corporation

• Helpful Hints for Analyzing in vitro Transcription/Translation Reactions on Polyacrylamide Gels

  By Natalie Betz, Ph.D.
  Promega Corporation

• Optimize Your TNT^® Reticulocyte Lysate Systems Reaction

  By Terri Sundquist, M.S.
  Promega Corporation

• Compatibility of GoTaq™ DNA Polymerase-Amplified PCR Products with TnT^® T7 Quick for PCR DNA

  By Natalie Betz, Ph.D.
  Promega Corporation

• Compatibility of the Pierce BCA Protein Assay with Promega Lysis Buffers and Lytic Assay Reagents

  By Natalie Betz, Ph.D.
  Promega Corporation

• AttoPhos^® AP Fluorescent Substrate and Western Blue^® Stabilized Substrate Detect Cytoplasmic Alkaline Phosphatase in NIH3T3 Cells  

  By Randy Hoffman, B.S., and Lisa DeBerg, M.S.
  Promega Corporation

• Characterization of TNT^® T7 Quick for PCR DNA

  By Natalie Betz, Ph.D.
  Promega Corporation

• A Comparison of pCI-neo Vector and pcDNA4/HisMax Vector

  By Brian Almond, Ph.D., and Elaine T. Schenborn, Ph.D.
  Promega Corporation

• Mass Spectrometric Analysis of MagneHis™ Purified Proteins

  By Robin Hurst, M.S., Gary Kobs, B.S., and Tonny Johnson, Ph.D.
  Promega Corporation

reverse transcription & cDNA synthesis

• Detecting pituitary adenylate cyclase-activating polypeptide in cartilaginous fish brain

  By Salvatore Valiante, Ph.D., and Marina Prisco, Ph.D.
  Department of Biological Sciences, Section of Evolutionary and Comparative Biology, University of Naples, Naples, Italy

• GoTaq^® Green Master Mix for Quick and Easy Two-Step RT-PCR

  By Kimberly Knoche, Ph.D., Mark Koeff, B.S., and Sarah Wheeler, B.S.
  Promega Corporation

• RNasin^® Plus RNase Inhibitor: New Protein for High Temperature RNase Inhibition

  By Christine Andrews, B.S., Fen Huang, B.S. and John Shultz, Ph.D.
  Promega Corporation

• Using Improm-II™ Reverse Transcription System for Two-Step RT-PCR

  By Katharine Miller, B.S.
  Promega Corporation

• Using ImProm-II™ Reverse Transcription System for Coupled RT-PCR

  By Katharine Miller, B.S.
  Promega Corporation

• Cloning Differential Display-PCR* Products with pGEM^®-T Easy Vector System

  By Rosalia Sirchia, Ph.D, Valentina Ciacciofera, and Claudio Luparello, Ph.D.
  Dipartimento di Biologia Cellulare e dello Svliuppo, Universita di Palermo, Italy

• Using ImProm-II™ Reverse Transcription System to Generate Fluorescent Probes

  By Natalie Betz, Ph.D., Randy Hoffman, B.S., Bing Hui Lin and Katharine Miller
  Promega Corporation

• Detection of Enteric Pathogenic Viruses in Shellfish by RT-PCR

  By O. Legeay, Ph.D., Y. Caudrelier, C. Cordevant, M.S., L. Rigottier-Gois, Ph.D., and M. Lange, Ph.D.
  Service R&D, Institut Pasteur de Lille, 1 rue de Professeur Calmette, BO 245, 59010 Lille Cedex, France

• Single-Cell RT-PCR Using RNasin^® Ribonuclease Inhibitor

  By Natalie Betz, Ph.D.
  Promega Corporation

• Use of M-MLV RT, RNase H-, Point Mutant, for mRNA-Differential Display Analysis of Parathyroid Hormone-Related Peptide (PTHrP)-Treated Breast Carcinoma Cells

By Claudio Luparello, Ph.D., Silvana Chimenti, B.S., Francesca Santamaria, B.S., Rosalia Sirchia, Ph.D., and Valentina Ciacciofera.
Departmento di Biologia Cellulare e dello Sviluppo, Universita di Palermo, Italy

• Single-Cell RT-PCR of Laser Capture-Microdissected Purkinje Cells

By Peter Benoehr¹, M.D., Christine Fleisch¹, Raffael Kurek², M.D., and Diethelm Wallwiener², M.D.
¹Department of Pharmacology, University of Tuebingen; ²Department of Obstetrics and Gynecology, University of Tuebingen, Germany

• Using the Access RT-PCR System: Reaction Parameters that Affect Efficient Amplification

By Daniel Kephart, Ph.D.
Promega Corporation

• Measuring Gene Expression from Mammalian Brain Tissue

By Daniel Kephart, Ph.D.
Promega Corporation

RNAi

• Human RNAi Promoters Work Effectively in Mouse NIH/3T3 Cells

  By Tom Yeager, Ph.D.
  Promega Corporation

• RNAi in Drosophila S2 Cells: Effect of dsRNA Size, Concentration, and Exposure Time

  By Natalie Betz, Ph.D.
  Promega Corporation

RNA purification

• Automated Poly(A)+ mRNA Isolation From Plant Tissues Using the PolyATtract^® System 1000

By Tracy Worzella, B.S. and Terri Grunst, B.S.
Promega Corporation

• Titration of Tomato Leaf Material Used as Starting Material for SV Total RNA Isolation

By Hemanth Shenoi, Ph.D., and Daniel Kephart, Ph.D.
Promega Corporation

• Can the SV RNA Isolation System be Used to Purify RNA from Frozen, Unprocessed Whole Blood?

By Daniel Kephart, Ph.D., and Hemanth Shenoi, Ph.D.
Promega Corporation

• Measuring Gene Expression from Mammalian Brain Tissue

By Daniel Kephart, Ph.D.
Promega Corporation

• A Simple and Efficient Method to Produce RNAs with Homogenous 3´ Ends  

By Cheng Kao, Ph.D.¹,  S. Rüdisser², Ph.D., and M. Zheng², Ph.D.
¹Department of Biology, Indiana University, Bloomington, IN 47405 USA; ²Department of Chemistry, University of California, Berkeley, and Lawrence Berkeley National Laboratory, Berkeley, CA 97420-1460 USA

signal transduction (& neuroscience)

• Specific Labeling of Neurons and Glia in Mixed Cerebrocortical Cultures

By Thomas Gillessen, Franz Tokai and Kati Breiter, Institut fuer Pharmakologie und Toxikologie, Sanitaetsakademie der Bundeswehr, Germany

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