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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

RNA Extraction from Formalin Fixed Paraffin Embedded (FFPE) Tissue Enabling Next Generation Detection of Gene Fusions

MDx

Tuesday, January 24, 2017

Dr. William Rehrauer
University Wisconsin-Madison

Learn about the detection of novel RNA fusions via new next generation sequencing library prep methods, automated extraction of total nucleic acid and the utility of this research in the field of molecular oncology.

To NanoDrop® or Not to NanoDrop®: Choosing the Most Appropriate Method for Nucleic Acid Quantitation

Genomics

Tuesday, February 14, 2017

Doug Wieczorek, PhD

Success or failure in DNA/RNA analysis applications often comes down to whether or not the appropriate amount of input nucleic acid is used, but nucleic acid quantitation methods reveal different information about a sample. This webinar will review absorbance, fluorescent nucleic acid-binding dyes and qPCR quantitation methods and present the advantages and disadvantages of each method. With this information, you'll be able to choose the appropriate quantitation method based on your sample type and downstream application.

MicroRNA Analysis Paired with Novel Cell Health Assays: A Complete Workflow

Genomics

Tuesday, February 28, 2017

Brad Hook, PhD

MicroRNAs (miRNAs) are small-noncoding RNA molecules that regulate gene expression post-transcriptionally. Amplification and overexpression of individual oncomiRs (miRNAs associated with cancer) or genetic loss of tumor suppressor miRNAs are associated with human cancer and are sufficient to drive tumorigenesis in mouse models. A global decrease in miRNA levels has been observed in human cancers and linked to genetic and epigenetic alterations in components of the miRNA biogenesis machinery. Learn more about how to analyze miRNAs in human cancer cells as we present data on a two studies that use new novel assays to monitor cell health and purify total RNA (including miRNAs) from the same cells.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Immune Checkpoint Bioassays Power Combination Immunotherapy

Drug Discovery

Tuesday, September 27, 2016

Richard Somberg, PhD

Therapeutic antibodies designed to block or activate immune checkpoint receptors are a powerful new approach to the treatment of cancer and other diseases. However, due to their biologic complexity the development of therapeutic antibodies requires unique functional and analytical tools. Here we present a suite of MOA-based functional bioassays for use in antibody screening, characterization, potency testing and stability studies.

Factors to Consider for Designing and Optimizing Assays Applied to 3D Cultures

Cell-Based Assays

Tuesday, September 13, 2016

Terry Riss, PhD

3D cell culture models present a challenge for using assay chemistries and imaging methods that were originally designed for measuring events from 2D monolayers of cells. In this webinar, we will present factors to consider when designing and optimizing assays applied to 3D cell culture models, case studies describing design of reagents to overcome the limitations inherent with detecting markers from large 3D culture models, and applications of novel real-time detection methods.

GTPase Biology: New Tools to the Rescue

Cell-Based Assays

Tuesday, August 23, 2016

Subhanjan Mondal, PhD

Cell Signaling is key to controlling cell health, and one key signaling node is GTPases. Technical options to analyze the biochemical activities of this incredibly important class of proteins are very limited. To support the needs of the research community, we will present various novel ways to analyze GTPase functions.

Introduction to Real-Time PCR: Basic Principles and Chemistries

Genomics

Tuesday, August 09, 2016

Leta Steffen, PhD

Real-Time PCR (qPCR) is the workhorse of most gene expression analysis. This webinar will provide an overview of basic qPCR principles compared to traditional PCR and common terms used in assay design and analysis.

What Factors Do You Need to Consider When Bringing Automation Into Your Lab?

Genomics

Tuesday, July 26, 2016

Shaun Peterson

Considering laboratory automation? Successful automation projects can increase efficiency by reducing failures, eliminating protocol bottlenecks and eliminating tedious tasks freeing lab members for other activities. Learn about laboratory automation, considerations for choosing automation and how Promega can assist you in automating nucleic acid extraction and downstream applications in your laboratory.

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