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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

MicroRNA Analysis Paired with Novel Cell Health Assays: A Complete Workflow

Genomics

Tuesday, February 28, 2017

Brad Hook, PhD

MicroRNAs (miRNAs) are small-noncoding RNA molecules that regulate gene expression post-transcriptionally. Amplification and overexpression of individual oncomiRs (miRNAs associated with cancer) or genetic loss of tumor suppressor miRNAs are associated with human cancer and are sufficient to drive tumorigenesis in mouse models. A global decrease in miRNA levels has been observed in human cancers and linked to genetic and epigenetic alterations in components of the miRNA biogenesis machinery. Learn more about how to analyze miRNAs in human cancer cells as we present data on a two studies that use new novel assays to monitor cell health and purify total RNA (including miRNAs) from the same cells.

Factors to Consider for Designing and Optimizing Assays Applied to 3D Cultures

Cell-Based Assays

Tuesday, March 14, 2017

Terry Riss, PhD

3D cell culture models present a challenge for using assay chemistries and imaging methods that were originally designed for measuring events from 2D monolayers of cells. In this webinar, we will present factors to consider when designing and optimizing assays applied to 3D cell culture models, case studies describing design of reagents to overcome the limitations inherent with detecting markers from large 3D culture models, and applications of novel real-time detection methods.

Optimizing and Qualifying Reporter Gene Bioassays for Immune Checkpoint Receptors

Drug Discovery

Tuesday, March 28, 2017

Zhijie Jey Cheng, PhD

Reporter gene assays for functional screening and development of antibody drug candidates are gaining popularity due to the significant advantages they offer over traditional methods that rely on human primary immune cells. We will discuss development of a series of MoA-based reporter gene bioassays for therapeutic antibodies targeting co-inhibitory receptors, co-stimulatory receptors and combination bioassays that target immune checkpoint receptors.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

The Key Role of FcR Activating Antibodies in Generating "Sterilizing" Immunity Against Herpes Simplex Virus

Drug Discovery

Tuesday, November 08, 2016

Betsy Herold, MD

Antibody dependent cell mediated cytotoxicity (ADCC) is an important immune effector mechanism against tumor and virus-infected cells. In recent years, ADCC has emerged as an important protective immune correlate in a number of vaccine studies for HIV and influenza. This webinar will highlight recent studies demonstrating the central role of FcR functions in vaccine-mediated protection against HSV-1 and HSV-2 infection, challenging the existing paradigm that high titer neutralizing antibodies are required.

Faster Mass Spec: Same-Day Sample Prep Now a Reality

Proteomics

Tuesday, October 25, 2016

Micheal M. Rosenblatt, PhD

Traditional protocols require digestion times of up to 18 hours, as well as reduction and alkylation prior to digestion. These denaturants often inhibit enzymes and modify proteins, and require removal prior to analysis. What if you could shorten digestion times to ~30 minutes, without denaturants or reductants or alkylating agents?

Immune Checkpoint Bioassays Power Combination Immunotherapy

Drug Discovery

Tuesday, September 27, 2016

Richard Somberg, PhD

Therapeutic antibodies designed to block or activate immune checkpoint receptors are a powerful new approach to the treatment of cancer and other diseases. However, due to their biologic complexity the development of therapeutic antibodies requires unique functional and analytical tools. Here we present a suite of MOA-based functional bioassays for use in antibody screening, characterization, potency testing and stability studies.

GTPase Biology: New Tools to the Rescue

Cell-Based Assays

Tuesday, August 23, 2016

Subhanjan Mondal, PhD

Cell Signaling is key to controlling cell health, and one key signaling node is GTPases. Technical options to analyze the biochemical activities of this incredibly important class of proteins are very limited. To support the needs of the research community, we will present various novel ways to analyze GTPase functions.

Introduction to Real-Time PCR: Basic Principles and Chemistries

Genomics

Tuesday, August 09, 2016

Leta Steffen, PhD

Real-Time PCR (qPCR) is the workhorse of most gene expression analysis. This webinar will provide an overview of basic qPCR principles compared to traditional PCR and common terms used in assay design and analysis.

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