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Promega Corporation

Plexor® Two-Step qRT-PCR System Technical Manual

Literature # TM264

The Plexor® qPCR System is a novel real-time PCR system for the quantitation of specific sequences within an RNA sample. This system uses ImProm-II™ Reverse Transcriptase for cDNA synthesis and the Plexor® qPCR technology for quantitation of any type of RNA sample, including total cellular RNA, poly(A)+ RNA or viral RNA. With this system, a separate reverse transcription reaction is assembled, and an aliquot of the resulting cDNA is added to an amplification reaction. This allows multiple amplifications to be performed using the same reverse transcription reaction.

Printed in USA. Revised 11/09.


PDF Icon Complete Protocol PDF (410kb)

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