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Promega Corporation

Plexor® One-Step qRT-PCR System Technical Manual

Literature # TM263

The Plexor® One-Step qRT-PCR System is a novel real-time PCR system for the quantitation of specific sequences within an RNA sample. This one-tube, two-enzyme system uses ImProm-II™ Reverse Transcriptase and the Plexor® qPCR technology for quantitation of any type of RNA sample, including total cellular RNA, poly(A)+ RNA or viral RNA. This system does not require a separate reverse transcription reaction. The reverse transcriptase and DNA polymerase are both added during the initial reaction setup, and the thermal cycler is programmed to perform the reverse transcription step, immediately followed by the thermal cycling program.

Printed in USA. Revised 11/09.

 

PDF Icon Complete Protocol PDF (418kb)

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