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Promega Corporation
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AMV Reverse Transcriptase

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Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

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  • *Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

AMV Reverse Transcriptase

300u
10u/μl M5101 $ 103.00 Add to cart

AMV Reverse Transcriptase

1,000u
10u/μl M5108 $ 275.00 Add to cart

AMV Reverse Transcriptase (HC)

600u
20–25u/μl M9004 $ 197.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, RT-PCR, first-strand cDNA synthesis.

Source

Purified Avian Myeloblastosis Virus particles.

Storage Buffer

200mM potassium phosphate (pH 7.2 at 4°C), 0.2% Triton® X-100, 2mM DTT and 50% gycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of dTTP into acid-insoluble form in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 40mM KCl, 8.75mM MgCl2, 10mM DTT, 0.1mg/ml acetylated BSA, 1mM radiolabeled dTTP and 0.25mM poly(A):oligo(dT).

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