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The Erase-a-Base™ System is designed for the rapid construction of plasmid or M13 subclones containing progressive unidirectional deletions of any inserted DNA. The system is based on the procedure developed by Henikoff, in which exonuclease III (Exo III) is used to specifically digest insert DNA from a 5´ protruding or blunt end restriction site. The adjacent sequencing primer binding site is protected from digestion by a 4-base 3´ overhang restriction site or by an α-phosphorothioate-filled end.
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We are sorry, but this product has been discontinued.
For a compatible replacement, contact Technical Service for assistance.
Erase-a-Base™ System (minus vectors & bacterial strain)
Erase-a-Base™ Control DNA
Exonuclease III 10X Buffer
S1 7.4X Buffer
S1 Stop Buffer
Klenow 1X Buffer
Ligase 10X Buffer
T4 DNA Ligase
DNA Polymerase I Large (Klenow) Fragment
Store at –20°C.
For product intended use please see Patents & Disclaimers tab.
E5750 For Research Use Only. Not for Use in Diagnostic Procedures.
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