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Promega Corporation

Flexi® Cloning System

The Flexi® Vector System is a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions without the need to resequence.

All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert.

Unlike site-specific recombination vector systems, the Flexi® Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of...

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

Flexi® System, Entry/Transfer

5 entry and 20 transfer reactions
- C8640 $ 285.00 Add to cart

Flexi® System, Transfer

100 transfer reactions
- C8820 $ 1,139.00 Add to cart

Carboxy Flexi® System, Transfer

50 transfer reactions
- C9320 $ 498.00 Add to cart

HaloTag® Cloning Starter System

1 each
- G6050 $ 356.00 Add to cart

Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

10X Flexi® Enzyme Blend (SgfI & PmeI)

- R1851 $ 109.00 Add to cart

10X Flexi® Enzyme Blend (SgfI & PmeI)

- R1852 $ 396.00 Add to cart

Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

- R1901 $ 108.00 Add to cart

Storage Conditions

Cat.# C8640 is comprised of Cat.# C8641 and A9280. Store Cat.# C8641 at –20°C; store Cat.# A9280 at room temperature. Store Cat.# C8820 and C9320 at –20°C. Store enzyme blends at –20°C.

For product intended use please see Patents & Disclaimers tab.

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