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Promega Corporation

Flexi® Cloning System

The Flexi® Vector System is a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions without the need to resequence.

All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert.

Unlike site-specific recombination vector systems, the Flexi® Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of...

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

Flexi® System, Entry/Transfer

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Flexi® System, Entry/Transfer

 Components

  • Wizard® SV Gel and PCR Clean-Up System

    A92801 x 10 preps
  • T4 DNA Ligase (HC)

    M179A1 x 500u
  • Nuclease-Free Water

    P119A1 x 1,250μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
  • 2X Flexi® Ligase Buffer

    R187A1 x 500μl
  • Flexi® Enzyme Blend (SgfI & PmeI)

    R188A2 x 50μl
5 entry and 20 transfer reactions
- C8640 $ 263.00 Add to cart

Flexi® System, Transfer

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Flexi® System, Transfer

 Components

  • T4 DNA Ligase (HC)

    M179A4 x 500u
  • Nuclease-Free Water

    P119A2 x 1,250μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
  • 2X Flexi® Ligase Buffer

    R187A2 x 500μl
  • Flexi® Enzyme Blend (SgfI & PmeI)

    R188A4 x 50μl
100 transfer reactions
- C8820 $ 1,053.00 Add to cart

Carboxy Flexi® System, Transfer

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Carboxy Flexi® System, Transfer

 Components

  • T4 DNA Ligase (HC)

    M179A2 x 500u
  • Nuclease-Free Water

    P119A1 x 1,250μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
  • 2X Flexi® Ligase Buffer

    R187A1 x 500μl
  • Flexi® Enzyme Blend (SgfI & PmeI)

    R188A1 x 50μl
  • Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

    R190A1 x 50μl
50 transfer reactions
- C9320 $ 461.00 Add to cart

HaloTag® Cloning Starter System

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HaloTag® Cloning Starter System

 Components

  • Wizard® SV Gel and PCR Clean-Up System

    A92801 x 10 preps
  • Flexi® System, Entry/Transfer

    C86411 x 1 each
  • HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack

    G37801 x 9 × 2μg
  • Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

    R19011 x 50μl
1 each
- G6050 $ 329.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

10X Flexi® Enzyme Blend (SgfI & PmeI)

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10X Flexi® Enzyme Blend (SgfI & PmeI)

  Components

  • Nuclease-Free Water

    P119A1 x 1,250μl
  • Flexi® Enzyme Blend (SgfI & PmeI)

    R185A1 x 25μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


25μl
- R1851 $ 101.00 Add to cart

10X Flexi® Enzyme Blend (SgfI & PmeI)

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10X Flexi® Enzyme Blend (SgfI & PmeI)

  Components

  • Nuclease-Free Water

    P119A2 x 1,250μl
  • 10X Flexi® Enzyme Blend (SgfI & PmeI)

    R185B1 x 100μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100μl
- R1852 $ 366.00 Add to cart

Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

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Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

  Components

  • Nuclease-Free Water

    P119A1 x 1,250μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
  • Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

    R190A1 x 50μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50μl
- R1901 $ 100.00 Add to cart

Storage Conditions

Cat.# C8640 is comprised of Cat.# C8641 and A9280. Store Cat.# C8641 at –20°C; store Cat.# A9280 at room temperature. Store Cat.# C8820 and C9320 at –20°C. Store enzyme blends at –20°C.

For product intended use please see Patents & Disclaimers tab.

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Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.