20mM Tris-HCl (pH 7.5), 2mM EGTA, 2mM EDTA, 1mM DTT, 10mM potassium phosphate (pH 7.5), 50% glycerol and 0.05% Triton® X-100.
PKC Activation 5X Buffer
1.6mg/ml phosphatidylserine, 0.16mg/ml diacylglycerol, 100mM Tris-HCl (pH 7.5), 50mM MgCl2.
PKC Coactivation 5X Buffer
1.25mM EGTA, 2mM CaCl2, 0.5mg/ml BSA.
Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.
For product intended use please see Patents & Disclaimers tab.