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Promega Corporation

PKC Kinase Enzyme System

See All Kinase Enzyme Systems
Native rat brain Protein Kinase C (PKC) is purified according to the method of Walton et al. (1). The purified PKC consists primarily of α, β and γ isoforms with lesser amounts of δ and ζ isoforms. The gene accession number for PKCα is NM_001105713.1.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase acti...

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PKC Kinase Enzyme System

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PKC Kinase Enzyme System

 Components

  • PKC Activation 5X Buffer

    V226A4 x 200μl
  • Protein Kinase C

    V526A1 x 0.5μg
  • Neurogranin(28–43) PKC Peptide Substrate

    V561A1 x 1mg
  • PKC Coactivation 5X Buffer

    V592A2 x 600μl
0.5μg
- V4504 $ 406.00 Add to cart

ADP-Glo™ Kinase Assay + PKC Kinase Enzyme System

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ADP-Glo™ Kinase Assay + PKC Kinase Enzyme System

 Components

  • PKC Kinase Enzyme System

    V45041 x 0.5μg
  • ADP-Glo™ Kinase Assay

    V91011 x 1,000 assays
1 each
- V4505 $ 808.00 Add to cart


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Storage Buffer

20mM Tris-HCl (pH 7.5), 2mM EGTA, 2mM EDTA, 1mM DTT, 10mM potassium phosphate (pH 7.5), 50% glycerol and 0.05% Triton® X-100.

PKC Activation 5X Buffer

1.6mg/ml phosphatidylserine, 0.16mg/ml diacylglycerol, 100mM Tris-HCl (pH 7.5), 50mM MgCl2.

PKC Coactivation 5X Buffer

1.25mM EGTA, 2mM CaCl2, 0.5mg/ml BSA.

Storage Conditions

Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

V4504, V4505 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V4505 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

V4505 U.S. Pat. No. 7,700,310 and other patents and patents pending.

V4505 U.S. Pat. No. 7,741,067 and other patents and patents pending.

V4505 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V4505 U.S. Pat. No. 8,183,007 and other patents pending.

V4505 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

V4505 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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