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Promega Corporation

CellTox™ Green Cytotoxicity Assay

The CellTox™ Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended to assess cytotoxicity in cell culture after experimental manipulation. The assay system uses a proprietary asymmetric cyanine dye that is excluded from viable cells but preferentially stains the DNA from dead cells. When the dye binds DNA released from cells, its fluorescence properties are substantially enhanced. Viable cells produce no appreciable increases in fluorescence. Therefore, the fluorescence signal produced by the binding interaction with dead cell DNA is prop...

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CellTox™ Green Cytotoxicity Assay

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CellTox™ Green Cytotoxicity Assay

 Components

  • Lysis Solution

    G182B1 x 0.5ml
  • Assay Buffer

    G610A1 x 10ml
  • CellTox™ Green Dye, 1,000X

    G873A1 x 20μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10ml
- G8741 $ 106.00 Add to cart

CellTox™ Green Cytotoxicity Assay

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CellTox™ Green Cytotoxicity Assay

 Components

  • Lysis Solution

    G182B1 x 0.5ml
  • Assay Buffer

    G610B1 x 50ml
  • CellTox™ Green Dye, 1,000X

    G873A5 x 20μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50ml
- G8742 $ 384.00 Add to cart

CellTox™ Green Cytotoxicity Assay

Close Window

CellTox™ Green Cytotoxicity Assay

 Components

  • Lysis Solution

    G182B1 x 0.5ml
  • Assay Buffer

    G610B2 x 50ml
  • CellTox™ Green Dye, 1,000X

    G873B1 x 200μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100ml
- G8743 $ 596.00 Add to cart

CellTox™ Green Express Cytotoxicity Assay

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CellTox™ Green Express Cytotoxicity Assay

 Components

  • CellTox™ Green Dye, 1,000X

    G873B1 x 200μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


200μl
- G8731 $ 566.00 Add to cart


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Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

10887MA_new_582pxFigure 1. CellTox™ Green Dye binds DNA of cells with impaired membrane integrity.

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CellTox™ Green Dye fluorescence is proportional to dead-cell number.Figure 2. CellTox™ Green Dye fluorescence is proportional to dead-cell number.
Schematic diagram of methods for delivering the CellTox™ Green Dye.Figure 3. Schematic diagram of methods for delivering the CellTox™ Green Dye.

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Cytotoxicity is concentration- and exposure period-dependent.Figure 4. Cytotoxicity is concentration- and exposure period-dependent.

Staurosporine was applied to K562 cells after introducing the CellTox™ Green Dye following the Express, No-Step Addition Method at Seeding Protocol. Fluorescence associated with the progression of cytotoxicity was measured after 24, 48 and 72 hours of cell exposure.

Multiplexing allows complementary measures of cell health.Figure 5. Multiplexing allows complementary measures of cell health.

CellTox™ Green Reagent was applied to bortezomib-treated K562 cells after 48 hours of exposure. Fluorescence associated with cytotoxicity was measured, then CellTiter-Glo® Reagent applied and bioluminescence associated with viability measured. These inverse measures produce similar EC50 values.

CellTox™ Green Reagent was applied to bortezomib-treated K562 cells after 48 hours of exposure. Fluorescence associated with cytotoxicity was measured, then CellTiter-Glo® Reagent applied and bioluminescence associated with viability measured. These inverse measures produce similar EC50 values.

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Use Restrictions

G8741, G8742, G8743, G8731 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G8741, G8742, G8743, G8731 Patent Pending.

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