Unit 6: Basics of Molecular Cloning (Blue/White Selection)

Molecular Cloning

This unit leads students through molecular cloning of DNA into a plasmid vector. Soon to come: A math-intensive protocol for cloning the luciferase gene using blue/white selection. Use the links below to access lectures, labs and writing assignments for this unit.

Lecture and Related Resources

Lecture Presentation

Basics of Molecular Cloning (PowerPoint® Lecture with notes; converted from PPT2007 presentation)

Related Cloning Resources

Other resources from Promega to help answer cloning-related questions.

The Answer is There in Blue and White

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More Science Education Resources

Training Support Program Biomath Other Multimedia from Promega

Other Units

Unit 1: The Relationship between Genes and Proteins Unit 2: The Chemistry of Inheritance Unit 3: Detecting Microbial Contamination Unit 4: Genomic DNA Purification Unit 5: Solving a Crime Using DNA Analysis

General Writing Resources for Scientists

A series of short articles covering grammar, style, usage and peer-review publication.

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On-site labs and classes

BioPharmaceutical Technology Center Institute