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The most sensitive assay available to quantify cytotoxicity due to loss of membrane integrity.

• Measure "dead-cell" protease biomarker leaked from cytoplasm into media.
• Loss of membrane integrity is a key indicator of cytotoxicity to determine the mechanism of cell death (necrosis).

The CytoTox-Glo™ Assay detects small changes in viability because of its high sensitivity. This graph shows the superior signal-to-noise ratios of the CytoTox-Glo™ Assay compared to a fluorescent LDH assay.
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• Detects as few as 200-500 cytotoxic cells in a total population of 10,000 cells.
• Detects less than 10 cytotoxic cells in a limiting dilution series.
• Scaleable to 384 and 1536 well plate formats.

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CytoTox-Glo™ Assay Chemistry

• Powered by Ultra-Glo™ Recombinant Luciferase.
• The amount of light generated correlates with the number of dead cells in a cell population.
• Eliminate fluorescent interference by compounds with a stable luminescence readout.

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• Homogeneous add-mix-measure protocol.
• Measure cytotoxicity and total cell number in the same well.

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• Ratiometric measures allow normalization for well-to-well variation from cell plating, compound addition or reagent dispensing.
• Robust cell-based assay chemistries are designed for multiplexing applications.