Issue 23
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Featured Interview
Pharmaceutical research and development in India |
177kb |
DPPIV Assay
Detection of dipeptidyl peptidase activity with DPPIV-Glo™ Assay |
301kb |
Stem Cells
Use of pluripotent stem cell-derived cardiomyocytes to understand mechanisms of cardiotoxic compounds |
153kb |
GPCR Assay
Monitoring the activity of G protein-coupled receptors (GPCRs) modulated by lipid or free fatty acid agonists |
249kb |
Cytochrome P450 Assays
CYP3A4 P450-Glo™ Assays with Luciferin-IPA: The most sensitive and selective bioluminescent CYP3A4 assay |
241kb |
Cytochrome P450 Assays
Six luminescent substrates for cytochrome P450 assays |
65kb |
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Issue 22
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HTS
High-Throughput Screening and Drug Discovery at St. Jude Children's Research Hospital |
276kb |
Screening
Screening for New Chemical Entities in Academic Settings |
142kb |
In Vivo Imaging
Non-invasive Bioluminescent Imaging Improves Understanding of Tumor Biology |
142kb |
GSH Assay
Homogeneous Luminescence-Based Assay for Quantifying the Glutathione Content in Mammalian Cells |
296kb |
Enzyme Assays
Luminogenic Enzyme Substrates: The Basis for a New Paradigm in Assay Design |
771kb |
Cell Culture
Doing Good Science: Authenticating Cell Line Identity |
225kb |
Cell Signaling
Studying MAP Kinase Signaling with a Small-Molecule Inhibitor, U0126 |
227kb |
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Issue 21
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TITLE
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PDF (size)
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Plasmid Purifcation
Transfection-Quality Plasmid DNA in As Little As Ten Minutes Using the PureYield™ Plasmid Miniprep System
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276kb |
Protein Expression
Selecting the Cell-Free Protein Expression System That Meets Your Experimental Goals
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611kb |
Protease Assay
New Bioluminescent Cell-Based Assays to Measure All Three Proteasome Protease Activities |
238kb |
Cell Signaling
Choosing the Right Assay to Monitor Your Signal Transduction Pathway |
903kb |
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Your Work: Citations of Recent Peer-Reviewed Literature
Subcellular Localization Using HaloTag® Techology; Cytochrome P450 CYP2C8 Assay; High-Throughput Luminescent β-Galactosidase Assay |
68kb |
Genetic Reporters
Improved Response Ratio for NF-κB Inhibition Analysis Using New Luciferase Reporter Vector (pGL4.32[luc2P/NFκB-RE/Hygro])
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152kb |
Gene Regulation
An Optimized System for Studying Transcriptional and Post-Transcriptional Gene Regulation |
227kb |
Reporter Assays
Luciferase Reporter Assays: Powerful, Adaptable Tools for Cell Biology Research
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660kb |
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Issue 20
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TITLE
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Science
Feature
A New Paradigm for Life Science
Research: Interview with Jeremy Caldwell, Ph.D.
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357kb |
Cell Viability
Monitoring Viability During
Gene-Directed Enzyme Prodrug Therapy with the CellTiter-Glo® Assay and Human Neural Progenitor Cells
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176kb |
Drug
Screening
Screening Traditional Chinese
Medicines Against Estrogen Receptors alpha and beta |
192kb |
RNAi
RNAi Knockdown in Primary Cells:
Suppression of Target Gene with psiSTRIKE™ and psiCHECK™ Vectors and
amaxa's Nucleofector® Technology |
992kb |
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ADME Screening
Detecting Toxicological Responses in
Cells with the Bioluminescent GSH-Glo™ Glutathione Assay |
269kb |
In Vitro Toxicology
Rat Hepatocyte Culture Physiology
Shows Enhanced Cytochrome P450 Activity on a Synthetic Extracellular
Matrix
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427kb |
Kinase Screening
Screen for Kinase Modulators in a
High-Throughput Format with Promega Kinase Reagents |
360kb |
Your Work
Citations of Recent Peer-Reviewed
Literature
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50kb |
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Cytotoxicity
High-Throughput Automation of
Multiplexed Cell-Based Assays for Viability and Cytotoxicity |
1679kb |
HTS
Application of Bioluminescence and
Cell-Based Assays in High-Throughput Screening Studies
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72kb |
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Issue 19
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TITLE
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PDF (size)
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microRNA
microRNA Biosensors: Application for
the psiCHECK™-2 Vector
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171kb |
Neuroscience
Neuroprotection: Understanding the
Modus Operandum of Glutamate Excitotoxicity
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66kb |
Apoptosis
New, Sensitive Bioluminescent
Caspase-Glo® 2 and 6 Assays: Using Inhibitors to Achieve Specificity in
Cells |
317kb |
Cell
Viability
Assessing Cell Viability in Primary
Cultures Using a Luminescent ATP-Based Assay: Applications for the
CellTiter-Glo® Assay |
99kb |
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Cytotoxicity
Using Protease Biomarkers to Measure
Viability and Cytotoxicity |
765kb |
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Issue 18
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TITLE
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PDF (size)
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Apoptosis
Using Caspase-Glo Assays to Perform
Whole Organism Screening for Apoptosis in Zebrafish Embryos
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158kb |
Calpain Assay
Screen for Calpain Inhibitors Using
a Cell-Based, High-Throughput Assay
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1716kb |
Custom Enzyme Assays
Custom Enzyme Substrates for
Luciferase-Based Assays |
131kb |
Apoptosis
Improve the Quality and Specificity of
Cell-Based Caspase-Glo® 8 and 9 Assays with a Protease Inhibitor |
157kb |
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Cell Viability
Measure Relative Numbers of Live and
Dead Cells and Normalize Assay Data to Cell Number |
525kb |
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Issue 17
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TITLE
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PDF (size)
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Luciferase Reporters
Enhanced Response Dynamics for
Transcription Analysis Using New pGL4 Luciferase Reporter Vectors
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135kb |
Luciferase Reporters
PIASy Regulation of STAT5 Activity: Different Reporter Vectors, Different
Responses
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155kb |
Technical
Tips
Normalizing Genetic Reporter
Assays: Approaches and Considerations for Increasing Consistency and
Statistical Significance |
130kb |
Instrumentation
Sensitive Detection of Reporter Gene Assay
Results |
194kb |
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Cell Viability
CellTiter-Glo® Luminescent Cell
Viability Assay: Application for Viability Studies of Cells Grown in Agarose |
263kb |
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Issue 16
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TITLE
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HTS Profiling
In Vitro Compound Profiling in 384-
and 1536-Well Formats: Using Bioluminescent ADME Assays to Understand In
Vivo Biology
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88kb |
Protease Assay
DPPIV-GLO™ Protease Assay: A More Sensitive
Method For Measuring GLY-PRO Cleaving Activity In Serum
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73kb |
HTS Cytotoxicity
Multiplexed Viability, Cytotoxicity And
Apoptosis Assays For Cell-Based Screening |
225kb |
Apoptosis
Timing Your Apoptosis Assays |
145kb |
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Luciferase Reporters
Using Luciferase Reporters to Screen for GPCR Modulators |
118kb |
Literature Review
Small-Molecule Kinase Inhibitors:
From Lab Bench to Clinic |
52kb |
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Science Feature
Capitalizing on China-Based Research
and Development |
92kb |
Cell Signaling
Promega Kinase Assay Products Flow Chart |
434kb |
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Issue 15
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TITLE
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PDF (size)
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Protease Assay
Monitoring Proteasome Activity with
a Cell-Based Assay Using a Single-Addition Luminescent Method
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315kb |
Protein Analysis
Reconstituting Endoplasmic
Reticulum-Associated Degradation (ERAD) in Rabbit Reticulocyte Lysate
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105kb |
Cytotoxicity
MultiTox-Fluor Multiplex
Cytotoxicity Assay Technology |
282kb |
On Press
From Our Scientists to Your Desk: External
Publications by Promega Authors |
43kb |
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Instrumentation
A Complete Solution for Measuring
Biological and Cellular Activities: GloMax Luminometers and Promega
Bioluminescent Assays |
192kb |
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Issue 14
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TITLE
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PDF (size)
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In vitro toxicology
The MAO-Glo™ Assay: A
Bioluminescent-Coupled Assay for Monoamine Oxidase Activity
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333kb |
Protein Analysis
HaloTag™ Technology: Cell Imaging and
Protein Analysis
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389kb |
Protein Analysis
HaloLink™ Resin for Protein
Pull-Down and Analysis |
322kb |
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In vitro toxicology
P450-Glo™ CYP2C19 and CYP2D6 Assays
and Screening Systems: The Method of Choice for in vitro P450 Assays |
332kb |
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Issue 13
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TITLE
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Chemical Profiling
The Biology of Chemical Space
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116kb |
GPCR Screening
Using Luciferase Assays to Study
G-Protein Coupled Receptor Pathways and Screen for GPCR Modulators
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241kb |
P450 Screening
Screen for Cytochrome P450
Activity Using a Luminescent Assay |
174kb |
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Kinase Assays
Choosing the Best Kinase Assay to
Meet Your Research Needs |
199kb |
HTS
Selecting Cell-Based Assays for Drug
Discovery Screening |
283kb |
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Chemical Profiling
NIH Forms Network of Molecular
Screening Centers to Identify Novel Chemical Probes for Life Science
Researchers |
199kb |
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Issue 12
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TITLE
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Cell Imaging
Perform Multicolor Live- and
Fixed-Cell Imaging Applications with HaloTag™ Interchangeable Labeling
Technology
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212kb |
Cell Signaling
Assay Virtually Any Kinase with Kinase-Glo®
Plus Luminescent Kinase Assay, A Homogeneous High-Throughput Assay
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272kb |
HTS
Perform Multiplexed Cell-Based Assays on
Automated Platforms |
178kb |
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Apoptosis
Citation Note: Development of a Homogeneous
Bioluminescent Caspase Assay |
105kb |
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Issue 11
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TITLE
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Labeling Cell Proteins
HaloTag™
Interchangeable Labeling
Technology for Cell Imaging and Protein Capture
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429kb |
Protease Assay
Assay for DPPIV Activity Using a
Homogeneous Luminescent Method
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252kb |
Puzzlement
Crossword Puzzle |
57kb |
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Protein Expression
Clone and Express Protein-Coding
Regions Using the Flexi®
Vector Systems |
312kb |
Cell Signaling
Citation Note: Measuring LPS-Induced PKC
Activity in U937 Cells |
57kb |
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Apoptosis
Citation Note: Detecting Caspase Activities in
Mouse Liver |
121kb |
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Issue 10
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TITLE
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Microbial Viability
Determining Microbial Viability Using a
Homogeneous Luminescent Assay
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292kb |
In Vitro Toxicology
Miniaturizing Luminescent P450-Glo™ Assays for
HTS
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149kb |
HTS Applications
Miniaturizing and Automating Cell Viability and
Reporter Assays for High-Throughput and Ultrahigh-Throughput Screening |
181kb |
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Cell-Based Assays
Multiplexing Homogeneous Cell-Based Assays |
208kb |
Cell Signaling
High-Throughput Screening Using a Universal
Luminescent Kinase Assay |
176kb |
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Issue
9
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TITLE
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Protein Purification
Finding the Right Protein Purification System
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219kb |
Protein Purification
Purifying His-Tagged Proteins from Insect and Mammalian
Cells
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162kb |
Just for Fun
Puzzlement: Proteins (crossword puzzle)
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92kb |
Apoptosis and Cell Viability
Characterizing Responses to Treatments Using Homogeneous
Caspase Activity and Cell Viability Assays
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145kb |
ddRNAi
Citation Note: ProInflammatory Phenotype of Coronary
Arteries Promotes Endothelial Apoptosis in Aging
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64kb |
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Issue
8
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Molecular Biology
Choosing the Right Luciferase to Light Your Way
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229kb |
Apoptosis
Citation Note: Using the Apo-ONE®
Assay in
Tissue Extracts And Using Luciferase Reporters to Study Hypoxia-Induced Gene
Expression
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84kb |
Apoptosis
Detect Caspase-8 and -9 Activities Using the
Homogeneous Caspase-Glo™ Assays
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160kb |
Just for Fun
Puzzlement: Cellular Analysis (crossword
puzzle)
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67kb |
Cell Signaling
Assay Protein Tyrosine Kinase and Protein
Tyrosine Phosphatase Activity in a Homogeneous, Non-Radioactive,
High-Throughput Format
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255kb |
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Issue
7
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TITLE
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In Vitro Toxicology
Screen for CYP450 Effects Using P450-Glo™ Luminescent
Cytochrome P450 Assay
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103kb |
Cell Signaling
Monitor Purified Phosphatase Activity with a
Homogeneous, Non-Radioactive High-Throughput Fluorogenic Assay
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112kb |
Apoptosis
Detecting Caspase Activity in
Staurosporine-Treated
Human Neuroblastoma Cells Using Fluorescent and Luminescent Methods
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91kb |
Molecular Biology
Knocking Down p53 Expression Using RNA
Interference
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80kb |
Neuroscience
Transfecting a Human Neuroblastoma Cell Line
with Monster Green™ Fluorescent Protein
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104kb |
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Issue
6
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TITLE
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Cell Viability
CellTiter-Glo® Assay: Application
for Assessing Direct Cytotoxicity and for Determining Cell Proliferation
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207kb |
In Vitro Toxicology
Choosing the Right Cell-Based Assay for Your
Research
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233kb |
Apoptosis
Caspase-Glo™ 3/7 Assay: Use Fewer Cells and
Spend Less Time with this Homogeneous Assay
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164kb |
Cellular Proteomics
Beta-Glo® Assay System: A
Luminescent β-Galactosidase Assay for Multiple Cell Types and Media
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113kb |
Cell Viability
Frequently Asked Questions
CytoTox-ONE™ Homogeneous Membrane Integrity Assay
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84kb |
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Issue
5
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TITLE
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Cell Signaling
ProFluor™ PKA Assay: Excellent Z-Factor Values Mean Reliable Results
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109kb |
Cell Signaling
Kinase-Glo™ Luminescent Kinase Assay: Detect Virtually Any Kinase
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140kb |
Cell Signaling
From One to 9,000 Samples: Using High-Density Streptavidin-Coated Membranes
for Kinase Detection
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112kb |
Cell Viability
The CellTiter-Blue™ Cell Viability Assay: Monitoring Cell Viability Using a
Fluorescent Redox Indicator Dye
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115kb |
Cell Viability
CellTiter-Glo™ Luminescent Cell Viability Assay for Cytotoxicity and Cell
Proliferation Studies
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118kb |
Neuroscience
Using Promega Antibodies for Neuroscience Research
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140kb |
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Issue 4
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TITLE
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Cell-Based Assays
In Vitro Toxicology and Cellular Fate Determination
Using Promega's Cell-Based Assays
(CellTiter-Glo™ Luminescent Cell Viability Assay, CytoTox 96®
Non-Radioactive Cytotoxicity Assay, Apo-ONE™ Homogeneous Caspase-3/7
Assay)
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110kb |
Cell-Based Assays
Introducing the CytoTox-ONE™ Homogeneous Membrane
Integrity Assay
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163kb |
Cell-Based Assays
CytoTox-ONE™ Homogeneous Membrane Integrity Assay:
A Tool for Automated Cytotoxicity Research
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107kb |
Cell-Based Assays
Apoptosis Detection Using the Acumen Explorer™
ADS and Promega's Anti-PARP p85 Fragment Antibody
(Anti-PARP p85 Fragment pAb)
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5.36MB |
Cell-Based Assays
Dual-Glo™ Luciferase Assay System: A
Homogeneous Dual-Reporter System
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107kb |
Announcement
ATCC Verifies Cell Line Identity Using Promega Reagents
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90kb |
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Issue 3
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TITLE
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Applications in Cytotoxicity
In Vitro Toxicology Assays Using
Promega Reagents and Systems
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78kb |
Applications in Cytotoxicity
Bringing Automation to the Cytotoxicity Researcher:
An ADME/Tox Application
(CytoTox 96® Non-Radioactive Cytotoxicity Assay)
|
111kb |
Applications in Cytotoxicity
The Predictive Nature of
High-Throughput Toxicity Screening
Using a Human Hepatocyte Cell Line
(CellTiter 96® AQueous One Solution Cell Proliferation Assay)
|
59kb |
Product Highlight
Promega's Anti-PARP p85 Fragment Antibody for
Apoptosis Detection
(Anti-PARP p85 Fragment pAb)
|
144kb |
Applications in Cell Biology
Using Terminal Deoxynucleotidyl Transferase
(TdT) Enzyme to
Detect TUNEL-Positive, GFP-Expressing Apoptotic Cells
(DeadEnd™ Fluorometric TUNEL System)
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60kb |
Product In-Cite: Promega Products in the Literature
Human ΔNp73 Regulates a Dominant Negative Feedback Loop
for TAp73 and p53
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24kb |
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Issue 2
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TITLE
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Applications in Cell Biology
The Apo-ONE™ Homogeneous Caspase-3/7 Assay:
A Simplified "Solution" for Apoptosis Detection
|
88kb |
Applications in Cell Biology
An Integrated Approach to Studying Apoptosis:
From Gene Expression to Cellular Events
(Apo-ONE™ Homogeneous Caspase-3/7 Assay; ImProm-II™ Reverse
Transcription System; SV Total RNA Isolation System)
|
138kb |
Applications in Cell Biology
A Herpes Simplex Virus Type 2 Protein
(ICP10 PK) Inhibits
Caspase-3 Activation in Hippocampal Neurons
(CaspACE™ FITC-VAD-FMK In Situ Marker)
|
105kb |
Product In-Cite: Promega Products in
the Literature
An Alternative, Non-Apoptotic Form of Programmed Cell Death
Sperandio, S. et al. (2000) Proc. Natl. Acad. Sci. USA 20,
14376–14381. (Apoptosis Detection System, Fluorescein)
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88kb |
Product In-Cite: Promega Products in
the Literature
Polycystin-1, the Gene Product of PKD1, Induces Resistance to Apoptosis and
Spontaneous Tubulogenesis in MDCK Cells
Boletta, A. et al. (2000) Mol. Cell. 6,
1267–1273.
(Apoptosis Detection System, Fluorescein)
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125kb |
New Product Highlight
CellTiter-Glo™ Luminescent Cell Viability Assay: A Sensitive and Rapid
Method for Determining Cell Viability
|
99kb |
New Product Highlight
High-Throughput Screening with the CellTiter-Glo™ Luminescent Cell
Viability Assay
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113kb |
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Issue 1
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TITLE
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Review
Scaffold Proteins in Mammalian JNK Signaling
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84kb |
Applications in Cell
Biology
Immunohistochemical Staining Using Promega Anti-ACTIVE® and Apoptosis
Antibodies
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388kb |
Apoptosis Detection
Flow Chart of Promega Apoptosis Products
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31kb |
Frequently Asked Questions
Kinase Inhibitors and Activators
|
53kb |
Product In-Cite: Promega Products
in the Literature
Store Operated Calcium Entry Activates at the GVBD Stage of Xenopus Meiosis
(SignaTECT® cdc2 Protein Kinase Assay System)
|
110kb |
Product In-Cite: Promega Products
in the Literature
Akt-Mediated Survival of Oligodendrocytes Induced by Neuregulins (CaspACE™
FITC-VAD-FMK In Situ Marker)
|
121kb |
Product In-Cite: Promega Products
in the Literature
The p44/42 Mitogen-Activated Protein Kinase Cascade is Involved in the Induction and
Maintenance of Astrocyte Stellation Mediated by Protein Kinase C (MEK Inhibitor U0126)
|
90kb |
New Products
Poly(A) mRNA Detection System
LigaFast™ Rapid DNA Ligation System
Shrimp Alkaline Phosphatase
FluoroTect™ GreenLys in vitro Translation Labeling System
Glo Lysis Buffer, 1X
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36kb |
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