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Six Bioluminescent Substrates for P450 Enzyme Assays

Promega Corporation

Luminogenic CYP assays use prosubstrates for the light-generating reaction of firefly luciferase. CYPs convert the prosubstrates to an active luciferin, which produces light in a second reaction with luciferase. The amount of light generated is proportional to the amount of luciferin produced by the CYP and, therefore, to CYP enzyme activity.

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Cell Notes 23 (2009) 19-20.
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