Monitoring the Activity of G Protein-Coupled Receptors (GPCRs) Modulated by Lipid or Free Fatty Acid Agonists
Said Goueli and Kevin Hsaio, Promega Corporation
We have recently demonstrated that the bioluminescent cAMP-Glo™ Assay can track direct activation of adenylate cyclase by
forskolin as well as agonists and antagonists that modulate GPCRs by monitoring the level of intracellular cAMP in the cell with
receptors that modulate Gαs (Dopamine D1) and those that modulate Gαi (Dopamine D2). Here we extend the application of the
cAMP-Glo™ Assay using receptors that are modulated by lipid and free fatty acid agonists. These receptors are of interest since
little is known about them and there is conflicting information in the available literature.
See information about
Cell Signaling Assay Reagents
from Promega
|
