|
Download
article
296kb
pdf? |
|
Homogeneous Luminescence-Based Assay for Quantifying the Glutathione Content in Mammalian Cells
Christiane Scherer, Silvia Christofanon, Mario Dicato, and Marc Diederich, LABORATOIRE DE BIOLOGIE
MOLÉCULAIRE ET CELLULAIRE DU CANCER, HÔPITAL KIRCHBERG, 9, RUE EDWARD STEICHEN, L-2540 LUXEMBURG,
LUXEMBURG
Promega has developed a new luminescence-based assay to rapidly quantify the total intra- and extracellular glutathione (GSH)
content of mammalian cells and tissues. This method is able to rapidly assess the basic level of this antioxidant, nonprotein thiol in
different eukaryotic cell lines. In addition, the antioxidant or prooxidant activity of diverse xenobiotics affecting cellular glutathione
levels can be investigated with little effort. Hence we describe experiments with the human leukemic cell line U937, where the
intracellular glutathione biosynthesis/level is induced or reduced after treatments with N-acetylcysteine (NAC) or buthionine-(S,R)-
sulfoxime (BSO), respectively.
|
