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Improved Response Ratio for NF-κB Inhibition Analysis Using New Luciferase Reporter
Vector (pGL4.32[Luc2P/NF-κB-RE/Hygro])
March Schumacher, Mario Dicato and Marc Diederich*, Laboratorie
de Biologie Moléculaire et Cellulaire du Cancer, Hôpital Kirchberg Luxembourg,
Luxemborg, *corresponding author
Promega has developed a new luciferase reporter vector for
single- or dual-reporter gene measurements. Our experiments were performed in order to inhibit TNFα-induced nuclear transcription factor kappa B
(NF-κB) activity by a natural product. Results with this new vector show improved reporter expression of firefly luciferase
luminescence compared to a competitor vector. This
new vector allows more accurate and precise measurement of NF-κB inhibition by
bioactive compounds paralleled by an economically
better consumption of the firefly luciferase vector.
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