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New Bioluminescent Cell-Based Assays to Measure All Three
Proteasome Protease Activities
Rich Moravec, Martha O'Brien, Bill Daily, Mike Scurria, Laurent
Bernad, Sandy Hagen, Alyssa TenHarmsel, Neal Cosby and Terry Riss, Promega
Corporation and Promega Biosciences, Inc.
Here we describe two new cell-based assays for the trypsin-like
and caspase-like protease activities of the proteaseome. These "add-mix-measure"
luminescent assays are sensitive enough to measure proteasome activity directly
in multiwell plates.
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