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RNAi Knockdown in Primary Cells: Suppression of Target Gene
with psiSTRIKE™ and psiCHECK™ Vectors and amaxa's Nucleofector® Technology
Efficient delivery of highly effective shRNAs into primary cells
enables studying gene function in a biologically relevant context. Here we provide an example of successful gene knockdown in primary cells using
an RNAi approach. Effective shRNAs were rapidly screened and selected using the psiCHECK™-2 Vector. Small hairpin
interfering RNAs were expressed from psiSTRIKE™–hMGFP Vectors that were efficiently delivered into primary cells
using amaxa’s Nucleofector® Technology.
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