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Screen for Calpain Inhibitors Using a Cell-Based,
High-Throughput Assay
Katheleen Seyb1,2, Jake Ni1, Mickey Huang1, Eli Schuman1, Mary
L. Michaelis2 and Marcie A. Glicksman1, 1Laboratory for Drug Discovery in
Neurodegeneration, Harvard Center for Neurodegeneration and Repair, Brigham and
Women's Hospital, 2Department of Pharmacology and Toxicology, University of
Kansas
We developed a cell-based assay for endogenous calpain activity using a cell-permeant
luminescent calpain substrate. Using the protocol described here, we were able
to identify potent, reproducible inhibitors of calpain activity in a
high-throughput screening application.
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