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Sensitive Detection of Reporter Gene Assay Results
Jason A. Bailey, Ph.D., Yuan-Wen Ge, Ph.D., and
Debomoy K. Lahiri, Ph.D.
Indiana University School of Medicine, Department of Psychiatry,
Institute of Psychiatric Research, Indianapolis, IN.
The number of primary cortical cells and neuronal populations
from cell lines becomes limiting when multiple treatments or conditions are
tested. Therefore assays and instrumentation used to study gene expression in
neuronal cell types need to be sensitive enough to work with limited cell
numbers. Here we describe and evaluate a reporter gene assay and instrumentation
for use in such cell populations.
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