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Using Luciferase Reporter Assays to Screen for GPCR Modulators
Aileen Paguio, M.S., Denise Garvin, M.S., Brad Swanson, Ph.D.,
Tracy Worzella, M.S., Keith Wood, Ph.D., and Frank Fan, Ph.D., Promega
Corporation
Previously, we described a strategy to construct stable cell
lines expressing two luciferases on two plasmids for a GPCR reporter assay. In
this report, we further demonstrate the utilities and advantages of the
dual-reporter luciferase GPCR assay through additional examples including an
agonist screen of a LOPAC compound collection.
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