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In Vitro Compound Profiling in 384- and 1536-Well Formats:
Using Bioluminescent ADME Assays to Understand In Vivo Biology
Tracy Worzella, M.S.1, James Cali, Ph.D.1,
Neal Cosby, Ph.D.1, Jean Shieh, B.S.2, Aoife Gallagher,
Ph.D.3, Kim Titus, B.A.4, Gerlinde Zerza-Schnitzhofer,
Ph.D.5, 1Promega Corporation, 2Labcyte
Corporation, 3Deerac Fluidics, 4Corning Inc. 5Tecan
AG Corporation
This article describes metabolic profiling applications in which
compounds are assayed against several different drug metabolizing enzymes on the
same assay plate. We obtain potency data for cytochrome P450 and monoamine
oxidase A using this parallel approach and dose-response testing of compounds
using both 384-well and 1536-well plate configurations.
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