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Monitoring Proteasome Activity with a Cell-Based Assay Using a
Single-Addition Luminescent Method
Rich Moravec, B.S.1, Martha O'Brien, Ph.D.1,
Bill Daily, Ph.D.2, Mike Scurria, B.S.2, Laurent Bernad,
Ph.D.2, Brad Larson, B.S.1, Tracy Worzella, M.S.1,
Kay Rashka, B.S.1, Jeri Culp, B.S.1, Brian McNamara, Ph.D.1,
and Terry Riss, Ph.D.1
1Promega Corporation, 2Promega Biosciences
Here we introduce the Proteasome-Glo™ Cell-Based Assay. This
"add-mix-measure" assay measures the chymotrypsin-like protease activity
associated with the proteasome in cultured cells.
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