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HaloLink™ Resin for Protein Pull-Down and Analysis
Marjeta Urh, Ph.D., Dan Simpson, Ph.D., Jacqui Sankbeil, M.S.,
Danette Hartzell, Ph.D., Natasha Karassina, M.S., Nadine Nassif, M.S., Jami
English, M.S., Ji Zhu, Ph.D., Poncho Meisenheimer, Ph.D., Dieter Klaubert,
Ph.D., Bob Bulliet, Ph.D. and Ketih V. Wood, Ph.D., Promega Corporation and Promega Biosciences,
Inc.
HaloLink™ Resin provides a method for specific, covalent and
oriented immobilization of proteins onto surfaces. Here we demonstrate the high
binding capacity and minimal nonspecific binding of the HaloLink™ Resin. We also
show that the covalent HaloTag™ bond provides stability during dilution and
stringent washing, minimizing the loss of the HaloTag™ fusion proteins from the
surface. By analyzing protein:protein interactions and enzyme activity for
several fusion proteins, we sho that HaloTag™ fusion proteins bound to the
HaloLink™ Resin maintain function. HaloLink™ Resin is not a purification resin;
however, in conjunction with protease cleavage, it can be used to isolate a
protein of interest.
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